Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi
Dr. Yan-long Yang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorShasha Zhang
Chinese Academy of Sciences Key Laboratory of Microbial, Physiological, and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorKe Ma
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorYuxing Xu
Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, China
Search for more papers by this authorQiaoqiao Tao
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorProf. Dr. Yihua Chen
State Key Laboratory of Microbial Resources, Yunnan Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorDr. Juan Chen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193 China
Search for more papers by this authorProf. Dr. Shunxing Guo
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193 China
Search for more papers by this authorDr. Jinwei Ren
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorDr. Wenzhao Wang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorProf. Dr. Yong Tao
Chinese Academy of Sciences Key Laboratory of Microbial, Physiological, and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorProf. Dr. Wen-Bing Yin
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Hongwei Liu
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorDr. Yan-long Yang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorShasha Zhang
Chinese Academy of Sciences Key Laboratory of Microbial, Physiological, and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorKe Ma
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorYuxing Xu
Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201 Yunnan, China
Search for more papers by this authorQiaoqiao Tao
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorProf. Dr. Yihua Chen
State Key Laboratory of Microbial Resources, Yunnan Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorDr. Juan Chen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193 China
Search for more papers by this authorProf. Dr. Shunxing Guo
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193 China
Search for more papers by this authorDr. Jinwei Ren
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorDr. Wenzhao Wang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorProf. Dr. Yong Tao
Chinese Academy of Sciences Key Laboratory of Microbial, Physiological, and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Search for more papers by this authorProf. Dr. Wen-Bing Yin
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Hongwei Liu
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorGraphical Abstract
Full circle: EriG, a member of the UbiA superfamily, was identified as the enzyme responsible for the cyclization of the cyathane skeleton in the mushroom Hericium erinaceum. Genome mining using the EriG protein sequence led to the identification of a new family of widespread UbiA-related diterpene cyclase genes in bacteria and fungi.
Abstract
Diterpene cyclases from bacteria and basidiomycete fungi are seldom studied. Here, we presented the identification and verification of EriG, a member of the UbiA superfamily, as the enzyme responsible for the cyclization of the cyathane skeleton in the mushroom Hericium erinaceum. Genome mining using the EriG protein sequence as a probe led to the discovery of a new family of ubiquitous UbiA-related diterpene cyclases in bacteria and fungi. We successfully characterized seven new diterpene cyclases from bacteria or basidiomycete fungi with the help of an engineered Escherichia coli strain and determined the structures of their corresponding products. A new diterpene with an unusual skeleton was generated during this process. The discovery of this new family of diterpene cyclases provides new insight into the UbiA superfamily.
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