Analysis of the Magnetic Properties of Nitrogenase FeMo Cofactor by Single-Crystal EPR Spectroscopy†
Dr. Thomas Spatzal
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie
BIOSS Centre for Biological Signalling Studies, Hebelstrasse 25, 79104 Freiburg (Germany)
Present address: Howard Hughes Medical Institute (HHMI) and Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena CA 91125 (USA)
Search for more papers by this authorCorresponding Author
Prof. Oliver Einsle
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie
BIOSS Centre for Biological Signalling Studies, Hebelstrasse 25, 79104 Freiburg (Germany)
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie===Search for more papers by this authorCorresponding Author
Prof. Susana L. A. Andrade
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie
BIOSS Centre for Biological Signalling Studies, Hebelstrasse 25, 79104 Freiburg (Germany)
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie===Search for more papers by this authorDr. Thomas Spatzal
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie
BIOSS Centre for Biological Signalling Studies, Hebelstrasse 25, 79104 Freiburg (Germany)
Present address: Howard Hughes Medical Institute (HHMI) and Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena CA 91125 (USA)
Search for more papers by this authorCorresponding Author
Prof. Oliver Einsle
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie
BIOSS Centre for Biological Signalling Studies, Hebelstrasse 25, 79104 Freiburg (Germany)
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie===Search for more papers by this authorCorresponding Author
Prof. Susana L. A. Andrade
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie
BIOSS Centre for Biological Signalling Studies, Hebelstrasse 25, 79104 Freiburg (Germany)
Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany) http://portal.uni-freiburg.de/biochemie===Search for more papers by this authorThis work was supported by Deutsche Forschungsgemeinschaft (grant AN 676/3 to S.L.A.A., grant EI 520/7 to O.E.) and the European Research Council.
Graphical Abstract
The catalytic center of nitrogenase, the [Mo:7Fe:9S:C]:homocitrate FeMo cofactor, is a S=3/2 system with a rhombic magnetic g tensor. Single-crystal EPR spectroscopy in combination with X-ray diffraction were used to determine the relative orientation of the g tensor with respect to the cluster structure. The protein environment influences the electronic structure of the FeMo cofactor, dictating preferred orientations of possible functional relevance.
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