Covalent Small-Molecule–RNA Complex Formation Enables Cellular Profiling of Small-Molecule–RNA Interactions†
Dr. Lirui Guan
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, 3A1, Jupiter, FL 33458 (USA)
Search for more papers by this authorCorresponding Author
Prof. Dr. Matthew D. Disney
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, 3A1, Jupiter, FL 33458 (USA)
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, 3A1, Jupiter, FL 33458 (USA)===Search for more papers by this authorDr. Lirui Guan
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, 3A1, Jupiter, FL 33458 (USA)
Search for more papers by this authorCorresponding Author
Prof. Dr. Matthew D. Disney
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, 3A1, Jupiter, FL 33458 (USA)
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, 3A1, Jupiter, FL 33458 (USA)===Search for more papers by this authorThis work was funded by the National Institutes of Health (RO1-GM079235) and The Scripps Research Institute. We thank S. G. Rzuczek for the synthesis of Ht azide used to procure 2H-4 derivatives. M.D.D. is a Camille & Henry Dreyfus Teacher–Scholar.
Graphical Abstract
Won't let you go! A strategy is described to design small molecules that react with their cellular RNA targets. This approach not only improves the activity of compounds targeting RNA in cell culture by a factor of about 2500 but also enables cell-wide profiling of its RNA targets.
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