Programmable Magnetic Tweezers and Droplet Microfluidic Device for High-Throughput Nanoliter Multi-Step Assays†
Dr. Anaïs Ali-Cherif
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
These authors contributed equally to this work.
Search for more papers by this authorDr. Stefano Begolo
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
Current address: California Institute of technology 1200 E. California Blvd. MC 101-20, Pasadena, CA 91125 (USA)
These authors contributed equally to this work.
Search for more papers by this authorDr. Stéphanie Descroix
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
Search for more papers by this authorDr. Jean-Louis Viovy
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
Search for more papers by this authorCorresponding Author
Dr. Laurent Malaquin
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)Search for more papers by this authorDr. Anaïs Ali-Cherif
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
These authors contributed equally to this work.
Search for more papers by this authorDr. Stefano Begolo
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
Current address: California Institute of technology 1200 E. California Blvd. MC 101-20, Pasadena, CA 91125 (USA)
These authors contributed equally to this work.
Search for more papers by this authorDr. Stéphanie Descroix
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
Search for more papers by this authorDr. Jean-Louis Viovy
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
Search for more papers by this authorCorresponding Author
Dr. Laurent Malaquin
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)
Institut Curie UMR 168, Research Center, CNRS, UMR168, 11 rue Pierre et Marie Curie, 75005 Paris (France)Search for more papers by this authorThis work was supported in part by EU project NADINE (FP7-NMP: 246513) and by the Equipex/Idex ANR program for the IPGG project. A.A.-C. acknowledges a PhD fellowship from the French Ministry of Defense; S.B. was supported by a Curie Institute International PhD fellowship.
Graphical Abstract
Tweezing out the answer: A microfluidic device combining droplets (less than 100 nL) and magnetic particles (see scheme) was implemented for fast heterogeneous multiplexed assays. Magnetic tweezers can perform the manipulations required in an immunoassay (capture, extraction, mixing, and rinsing). This method was applied to the diagnosis of congenital hypothyroidism with 14 pM sensitivity.
Supporting Information
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