Communication

Efficient Tryptic Proteolysis Accelerated by Laser Radiation for Peptide Mapping in Proteome Analysis^†

Guoping Yao

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (China), Fax: (+86) 21-6564-1740

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Prof. Chunhui Deng,

Prof. Chunhui Deng

[email protected]

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (China), Fax: (+86) 21-6564-1740

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Prof. Xiangmin Zhang,

Prof. Xiangmin Zhang

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (China), Fax: (+86) 21-6564-1740

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Prof. Pengyuan Yang,

Prof. Pengyuan Yang

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (China), Fax: (+86) 21-6564-1740

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Guoping Yao,

Guoping Yao

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (China), Fax: (+86) 21-6564-1740

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Prof. Chunhui Deng,

Prof. Chunhui Deng

[email protected]

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (China), Fax: (+86) 21-6564-1740

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Prof. Xiangmin Zhang,

Prof. Xiangmin Zhang

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (China), Fax: (+86) 21-6564-1740

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Prof. Pengyuan Yang,

Prof. Pengyuan Yang

Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (China), Fax: (+86) 21-6564-1740

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First published: 24 September 2010

https://doi.org/10.1002/anie.201004152

Citations: 21

^†

This research was supported by the National Natural Science Foundation of China (Projects: 20875017, 21075022, and 20735005), the Technological Innovation Program of Shanghai (09JC1401100), the National Basic Research Priorities Program (Project: 2007CB914100/3), and the Shanghai Leading Academic Discipline Project (B109).

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Graphical Abstract

Back to basics: Coupled with MALDI-TOF MS, laser-assisted proteolysis (see schematic illustration) enabled rapid protein digestion and peptide mapping without the need for enzyme immobilization to increase the efficiency of tryptic digestion. Protein solutions containing trypsin were digested in less than a minute upon irradiation at 808 nm with a laser.

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Volume49, Issue44

October 25, 2010

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Efficient Tryptic Proteolysis Accelerated by Laser Radiation for Peptide Mapping in Proteome Analysis^†

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Efficient Tryptic Proteolysis Accelerated by Laser Radiation for Peptide Mapping in Proteome Analysis†

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Efficient Tryptic Proteolysis Accelerated by Laser Radiation for Peptide Mapping in Proteome Analysis^†