A Straightforward Approach for Cellular-Uptake Quantification†
Dr. David Paramelle
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
These authors contributed equally.
Search for more papers by this authorDr. Gilles Subra
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
These authors contributed equally.
Search for more papers by this authorLubomir L. Vezenkov
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. Marie Maynadier
Institut de Recherche en Cancérologie CRLC, INSERM Unité 826, 208 rue des Apothicaires, 34298 Montpellier Cedex 1 (France)
Search for more papers by this authorChristophe André
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorProf. Christine Enjalbal
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. Monique Calmès
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. Marcel Garcia
Institut de Recherche en Cancérologie CRLC, INSERM Unité 826, 208 rue des Apothicaires, 34298 Montpellier Cedex 1 (France)
Search for more papers by this authorProf. Jean Martinez
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. Muriel Amblard
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. David Paramelle
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
These authors contributed equally.
Search for more papers by this authorDr. Gilles Subra
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
These authors contributed equally.
Search for more papers by this authorLubomir L. Vezenkov
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. Marie Maynadier
Institut de Recherche en Cancérologie CRLC, INSERM Unité 826, 208 rue des Apothicaires, 34298 Montpellier Cedex 1 (France)
Search for more papers by this authorChristophe André
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorProf. Christine Enjalbal
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. Monique Calmès
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. Marcel Garcia
Institut de Recherche en Cancérologie CRLC, INSERM Unité 826, 208 rue des Apothicaires, 34298 Montpellier Cedex 1 (France)
Search for more papers by this authorProf. Jean Martinez
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorDr. Muriel Amblard
Institut des Biomolécules Max Mousseron (IBMM), UMR5247 CNRS, Universités Montpellier 1 et 2, 15 avenue Charles Flahault, 34000 Montpellier (France), Fax: (+33) 4-6754-8654 http://www.ibmm.univ-montp1.fr/
Search for more papers by this authorThis study was supported in part by the French National Agency (ANR) (ANR-08-BLAN-0066-01) and a grant from the Università di Napoli Federico II, Italy (L.L.V.).
Graphical Abstract
Putting a number on it: MALDI-TOF MS enabled direct quantification of the cellular uptake of cell-penetrating peptides (CPPs) by MDA-MB-231 breast cancer cells. This sensitive general strategy (see schematic representation), which requires no purification or separation steps, relies on the enhancement and discrimination of the MS signals of an α-cyano-4-hydroxycinnamic acid tag in a neutral α-cyano-4-hydroxycinnamic methyl ester matrix.
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