Vesicle Tubulation with Self-Assembling DNA Nanosprings
Michael W. Grome
Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516 USA
Search for more papers by this authorDr. Zhao Zhang
Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516 USA
Search for more papers by this authorDr. Frédéric Pincet
Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516 USA
Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University, Université Paris Diderot Sorbonne Paris Cité, Sorbonne Universités UPMC Univ Paris 06, CNRS, 24 rue Lhomond, 75005 Paris, France
Search for more papers by this authorCorresponding Author
Prof. Chenxiang Lin
Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516 USA
Search for more papers by this authorMichael W. Grome
Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516 USA
Search for more papers by this authorDr. Zhao Zhang
Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516 USA
Search for more papers by this authorDr. Frédéric Pincet
Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516 USA
Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University, Université Paris Diderot Sorbonne Paris Cité, Sorbonne Universités UPMC Univ Paris 06, CNRS, 24 rue Lhomond, 75005 Paris, France
Search for more papers by this authorCorresponding Author
Prof. Chenxiang Lin
Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516 USA
Search for more papers by this authorAbstract
A major goal of nanotechnology and bioengineering is to build artificial nanomachines capable of generating specific membrane curvatures on demand. Inspired by natural membrane-deforming proteins, we designed DNA-origami curls that polymerize into nanosprings and show their efficacy in vesicle deformation. DNA-coated membrane tubules emerge from spherical vesicles when DNA-origami polymerization or high membrane-surface coverage occurs. Unlike many previous methods, the DNA self-assembly-mediated membrane tubulation eliminates the need for detergents or top-down manipulation. The DNA-origami design and deformation conditions have substantial influence on the tubulation efficiency and tube morphology, underscoring the intricate interplay between lipid bilayers and vesicle-deforming DNA structures.
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