A Telomerase-Specific Doxorubicin-Releasing Molecular Beacon for Cancer Theranostics
Yi Ma
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorZhaohui Wang
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorMin Zhang
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorZhihao Han
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorDan Chen
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorQiuyun Zhu
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorWeidong Gao
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Zhiyu Qian
Department of Biomedical Engineering, School of Automation, Nanjing University of Aeronautics and Astronautics, 29 Yudao Street, Nanjing, 210016 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Yueqing Gu
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorYi Ma
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorZhaohui Wang
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorMin Zhang
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorZhihao Han
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorDan Chen
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorQiuyun Zhu
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorWeidong Gao
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Zhiyu Qian
Department of Biomedical Engineering, School of Automation, Nanjing University of Aeronautics and Astronautics, 29 Yudao Street, Nanjing, 210016 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Yueqing Gu
State Key Laboratory of Natural Medicines, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Road, Nanjing, 210009 China
Search for more papers by this authorAbstract
A molecular beacon-based drug delivery system was designed for both detection of telomerase activity in living cells and telomerase-triggered drug release for precise cancer treatment. This system is composed of a gold nanoparticle core densely packed with FITC-labeled hairpin DNA sequences hybridized with telomerase primers. Molecules of the anticancer drug doxorubicin were intercalated into the stem region of the DNA sequence. The presence of telomerase will elongate the primers, leading to inner chain substitution followed by the release of the FITC fluorescence and the trapped doxorubicin. This molecular beacon could specifically distinguish tumor cells and normal cells based on telomerase activity, precisely release doxorubicin in response to telomerase activity in the tumor cells, and prevent toxicity to normal organs.
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