Monitoring of Endogenous Hydrogen Sulfide in Living Cells Using Surface-Enhanced Raman Scattering
Corresponding Author
Dr. Da-Wei Li
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
These authors contributed equally to this work.
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)Search for more papers by this authorLu-Lu Qu
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
These authors contributed equally to this work.
Search for more papers by this authorKai Hu
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
Search for more papers by this authorCorresponding Author
Prof. Dr. Yi-Tao Long
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)Search for more papers by this authorProf. Dr. He Tian
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
Search for more papers by this authorCorresponding Author
Dr. Da-Wei Li
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
These authors contributed equally to this work.
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)Search for more papers by this authorLu-Lu Qu
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
These authors contributed equally to this work.
Search for more papers by this authorKai Hu
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
Search for more papers by this authorCorresponding Author
Prof. Dr. Yi-Tao Long
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)Search for more papers by this authorProf. Dr. He Tian
Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China)
Search for more papers by this authorAbstract
Hydrogen sulfide (H2S) has emerged as an important gasotransmitter in diverse physiological processes, although many aspects of its roles remain unclear, partly owing to a lack of robust analytical methods. Herein we report a novel surface-enhanced Raman scattering (SERS) nanosensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA), for detecting the endogenous H2S in living cells. The detection is accomplished with SERS spectrum changes of AuNPs/4-AA resulting from the reaction of H2S with 4-AA on AuNPs. The SERS nanosensor exhibits high selectivity toward H2S. Furthermore, AuNPs/4-AA responds to H2S within 1 min with a 0.1 μM level of sensitivity. In particular, our SERS method can be utilized to monitor the endogenous H2S generated in living glioma cells, demonstrating its great promise in studies of pathophysiological pathways involving H2S.
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