Discovery of Hepatitis C Virus NS3 Helicase Inhibitors by a Multiplexed, High-Throughput Helicase Activity Assay Based on Graphene Oxide†
Hongje Jang
Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
Search for more papers by this authorSoo-Ryoon Ryoo
Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)
Search for more papers by this authorYoung-Kwan Kim
Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)
Search for more papers by this authorSoojin Yoon
Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701 (Korea)
Search for more papers by this authorHenna Kim
Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
Search for more papers by this authorProf. Dr. Sang Woo Han
Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
Search for more papers by this authorProf. Dr. Byong-Seok Choi
Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
Search for more papers by this authorProf. Dr. Dong-Eun Kim
Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701 (Korea)
Search for more papers by this authorCorresponding Author
Prof. Dr. Dal-Hee Min
Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)
Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)===Search for more papers by this authorHongje Jang
Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
Search for more papers by this authorSoo-Ryoon Ryoo
Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)
Search for more papers by this authorYoung-Kwan Kim
Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)
Search for more papers by this authorSoojin Yoon
Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701 (Korea)
Search for more papers by this authorHenna Kim
Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
Search for more papers by this authorProf. Dr. Sang Woo Han
Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
Search for more papers by this authorProf. Dr. Byong-Seok Choi
Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
Search for more papers by this authorProf. Dr. Dong-Eun Kim
Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701 (Korea)
Search for more papers by this authorCorresponding Author
Prof. Dr. Dal-Hee Min
Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)
Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)===Search for more papers by this authorThis work was supported by the Basic Science Research Program (2011-0017356 for D.-H. M., 2009-0092818, 2011-0020322 for B.-S. C. and 2010-0019306, 2011-0016385 for D.-E. K.), the EPB Center (2008-0062042), and by the Research Center Program (EM1202) of the Institute for Basic Science (D.-H. M.) through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST).
Graphical Abstract
Ein einfaches Testsystem auf der Basis von Graphenoxid (GO) ermöglicht das Screening selektiver Inhibitoren einer Helicase des Hepatitis-C-Virus (HCV) zusammen mit Inhibitoren eines SARS-Coronavirus (SARS-CoV) (siehe Schema). In einem einzelnen Screen wurden fünf hochselektive Inhibitoren der HCV-Helicase gefunden, die ortholog zur SARS-CoV-Helicase sind. Einige dieser Treffer wurden mit dem gleichen GO-Assay validiert.
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