Solution NMR Structure of Proteorhodopsin†
Sina Reckel
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
These authors contributed equally to this work.
Search for more papers by this authorDaniel Gottstein
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
These authors contributed equally to this work.
Search for more papers by this authorJochen Stehle
Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)
Search for more papers by this authorDr. Frank Löhr
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Search for more papers by this authorDr. Mirka-Kristin Verhoefen
Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)
Search for more papers by this authorDr. Mitsuhiro Takeda
Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan)
Search for more papers by this authorRobert Silvers
Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)
Search for more papers by this authorProf. Dr. Masatsune Kainosho
Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan)
Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)
Search for more papers by this authorProf. Dr. Clemens Glaubitz
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Search for more papers by this authorProf. Dr. Josef Wachtveitl
Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)
Search for more papers by this authorDr. Frank Bernhard
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Search for more papers by this authorProf. Dr. Harald Schwalbe
Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)
Search for more papers by this authorProf. Dr. Peter Güntert
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Frankfurt Institute for Advanced Studies, Goethe University Frankfurt, Ruth-Moufang-Str.1, 60438 Frankfurt am Main (Germany)
Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)
Search for more papers by this authorCorresponding Author
Prof. Dr. Volker Dötsch
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this authorSina Reckel
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
These authors contributed equally to this work.
Search for more papers by this authorDaniel Gottstein
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
These authors contributed equally to this work.
Search for more papers by this authorJochen Stehle
Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)
Search for more papers by this authorDr. Frank Löhr
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Search for more papers by this authorDr. Mirka-Kristin Verhoefen
Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)
Search for more papers by this authorDr. Mitsuhiro Takeda
Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan)
Search for more papers by this authorRobert Silvers
Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)
Search for more papers by this authorProf. Dr. Masatsune Kainosho
Structural Biology Research Center, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 (Japan)
Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)
Search for more papers by this authorProf. Dr. Clemens Glaubitz
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Search for more papers by this authorProf. Dr. Josef Wachtveitl
Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue Str. 7, 60438 Frankfurt (Germany)
Search for more papers by this authorDr. Frank Bernhard
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Search for more papers by this authorProf. Dr. Harald Schwalbe
Institute for Organic Chemistry and Chemical Biology and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 7–9, 60438 Frankfurt (Germany)
Search for more papers by this authorProf. Dr. Peter Güntert
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Frankfurt Institute for Advanced Studies, Goethe University Frankfurt, Ruth-Moufang-Str.1, 60438 Frankfurt am Main (Germany)
Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397 (Japan)
Search for more papers by this authorCorresponding Author
Prof. Dr. Volker Dötsch
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)
Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt (Germany)Search for more papers by this authorThis work was supported by the Deutsche Forschungsgemeinschaft (grant number DO545/7-1 and SFB 807), the European Drug Initiative on Channels and Transporters (EDICT) contract number HEALTH-F4-2007-201924, the NIH (grant number U54 GM094608), the Center for Biomolecular Magnetic Resonance (BMRZ) and the Cluster of Excellence Frankfurt (Macromolecular Complexes). The [11,20-13C2]retinal was a kind gift of Johan Lugtenburg, Peter Verdegem (Leiden University), Neville McLean, Malcolm H. Levitt and Richard C. D. Brown (Southampton University). P.G. gratefully acknowledges financial support by the Lichtenberg program of the Volkswagen Foundation and by a Grant-in-Aid for Scientific Research of the Japan Society for the Promotion of Science.
Graphical Abstract
Ein gelöstes Rätsel: Die mit Lösungs-NMR-Spektroskopie bestimmte Struktur der aus sieben transmembranären Helices gebildeten Protonenpumpe Proteorhodopsin wurde durch Kombination von Daten aus NOE-Experimenten und verstärkter paramagnetischer Relaxation erhalten (siehe Bild). Die Genauigkeit, mit der die Struktur aufgeklärt wurde, konnte durch dipolare Restkopplungen verbessert werden.
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