Tracking N-Acetyllactosamine on Cell-Surface Glycans In Vivo†
Tianqing Zheng
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
Search for more papers by this authorDr. Hao Jiang
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
Search for more papers by this authorDr. Marilyn Gros
Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorDr. David Soriano del Amo
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
Search for more papers by this authorSubha Sundaram
Department of Cell Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorProf. Grégoire Lauvau
Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorProf. Florence Marlow
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorDr. Yi Liu
The Molecular Foundry, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley (USA)
Search for more papers by this authorProf. Pamela Stanley
Department of Cell Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorCorresponding Author
Prof. Peng Wu
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022Search for more papers by this authorTianqing Zheng
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
Search for more papers by this authorDr. Hao Jiang
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
Search for more papers by this authorDr. Marilyn Gros
Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorDr. David Soriano del Amo
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
Search for more papers by this authorSubha Sundaram
Department of Cell Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorProf. Grégoire Lauvau
Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorProf. Florence Marlow
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorDr. Yi Liu
The Molecular Foundry, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley (USA)
Search for more papers by this authorProf. Pamela Stanley
Department of Cell Biology, Albert Einstein College of Medicine of Yeshiva University (USA)
Search for more papers by this authorCorresponding Author
Prof. Peng Wu
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022
Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461 (USA), Fax: (+1) 718-678-1022Search for more papers by this authorThis work was supported by the National Institutes of Health (R01GM093282 to P.W. and R01CA036434 to P.S.) and the Mizutani Foundation for Glycoscience (to P.W.). Y.L. was supported by the Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under contract No. DE-AC02-05 CH11231. We thank Prof. Carolyn Bertozzi for providing DIFO-647. Two disaccharides used in the kinetic assay were provided by The Consortium for Functional Glycomics (funded by NIGMS-GM62116).
Graphical Abstract
Viele Säugetier-Glycane, die mit Signalrezeptoren assoziiert sind, enthalten N-Acetyllactosamin an der letzten oder vorletzten Position. Eine hochspezifische Methode zur Markierung dieses Disaccharids auf Zelloberflächen-Glycoproteinen lebender, kultivierter Zellen und Zebrafischembryos nutzt einen zweistufigen chemoenzymatischen Ansatz aus In-situ-Fucosylierung und bioorthogonaler Klickreaktion (siehe Schema; α(1,3)FucT=α(1,3)-Fucosyltransferase).
Supporting Information
Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors.
| Filename | Description |
|---|---|
| ange_201100265_sm_miscellaneous_information.pdf547.2 KB | miscellaneous_information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
References
- 1H. H. Freeze, Nat. Rev. Genet. 2006, 7, 537–551.
- 2S. T. Laughlin, C. R. Bertozzi, Proc. Natl. Acad. Sci. USA 2009, 106, 12–17.
- 3J. M. Baskin, C. R. Bertozzi, QSAR Comb. Sci. 2007, 26, 1211–1219.
- 4S. R. Hanson, T. L. Hsu, E. Weerapana, K. Kishikawa, G. M. Simon, B. F. Cravatt, C. H. Wong, J. Am. Chem. Soc. 2007, 129, 7266–7267.
- 5D. Soriano del Amo, W. Wang, H. Jiang, C. Besanceney, A. C. Yan, M. Levy, Y. Liu, F. L. Marlow, P. Wu, J. Am. Chem. Soc. 2010, 132, 16893–16899.
- 6A. Varki, R. D. Cummings, J. D. Esko, H. H. Freeze, P. Stanley, C. R. Bertozzi, G. W. Hart, M. E. Etzler, Essentials of Glycobiology, 2nd ed., Cold Spring Harbor, New York, 2008.
- 7K. S. Lau, E. A. Partridge, A. Grigorian, C. I. Silvescu, V. N. Reinhold, M. Demetriou, J. W. Dennis, Cell 2007, 129, 123–134.
- 8R. S. Haltiwanger, J. B. Lowe, Annu. Rev. Biochem. 2004, 73, 491–537.
- 9T. Ichikawa, J. Nakayama, N. Sakura, T. Hashimoto, M. Fukuda, M. N. Fukuda, T. Taki, J. Histochem. Cytochem. 1999, 47, 1593–1602.
- 10W. Wang, T. Hu, P. A. Frantom, T. Zheng, B. Gerwe, D. S. Del Amo, S. Garret, R. D. Seidel III, P. Wu, Proc. Natl. Acad. Sci. USA 2009, 106, 16096–16101.
- 11G. Srivastava, K. J. Kaur, O. Hindsgaul, M. M. Palcic, J. Biol. Chem. 1992, 267, 22356–22361.
- 12J. C. Jewett, C. R. Bertozzi, Chem. Soc. Rev. 2010, 39, 1272–1279.
- 13S. W. Lin, T. M. Yuan, J. R. Li, C. H. Lin, Biochemistry 2006, 45, 8108–8116.
- 14S. K. Patnaik, P. Stanley, Methods Enzymol. 2006, 416, 159–182.
- 15S. J. North, H. H. Huang, S. Sundaram, J. Jang-Lee, A. T. Etienne, A. Trollope, S. Chalabi, A. Dell, P. Stanley, S. M. Haslam, J. Biol. Chem. 2010, 285, 5759–5775.
- 16M. Eckhardt, B. Gotza, R. Gerardy-Schahn, J. Biol. Chem. 1998, 273, 20189–20195.
- 17J. C. Jewett, E. M. Sletten, C. R. Bertozzi, J. Am. Chem. Soc. 2010, 132, 3688–3690.
- 18S. Oelmann, P. Stanley, R. Gerardy-Schahn, J. Biol. Chem. 2001, 276, 26291–26300.
- 19J. M. Baskin, J. A. Prescher, S. T. Laughlin, N. J. Agard, P. V. Chang, I. A. Miller, A. Lo, J. A. Codelli, C. R. Bertozzi, Proc. Natl. Acad. Sci. USA 2007, 104, 16793–16797.
- 20S. K. Patnaik, A. Zhang, S. Shi, P. Stanley, Arch. Biochem. Biophys. 2000, 375, 322–332.
- 21M. Lanteri, V. Giordanengo, N. Hiraoka, J. G. Fuzibet, P. Auberger, M. Fukuda, L. G. Baum, J. C. Lefebvre, Glycobiology 2003, 13, 909–918.
- 22H. Lis, N. Sharon, Methods Enzymol. 1987, 138, 544–551.
- 23M. S. Nachbar, J. D. Oppenheim, J. O. Thomas, J. Biol. Chem. 1980, 255, 2056–2061.
- 24S. T. Laughlin, J. M. Baskin, S. L. Amacher, C. R. Bertozzi, Science 2008, 320, 664–667.
Citing Literature
This is the
German version
of Angewandte Chemie.
Note for articles published since 1962:
Do not cite this version alone.
Take me to the International Edition version with citable page numbers, DOI, and citation export.
We apologize for the inconvenience.
