Volume 38, Issue 13-14 pp. 2000-2004
Communication

Interaction of a PDZ Protein Domain with a Synthetic Library of All Human Protein C Termini

Ulrich Hoffmüller

Ulrich Hoffmüller

Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität Berlin, Schumannstrasse 20/21, D-10098 Berlin (Germany), Fax: (+49) 30-2802-6460

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Michael Russwurm

Michael Russwurm

Institut für Pharmakologie, Freie Universität Berlin, Thielallee 69–73, D-14195 Berlin (Germany)

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Frank Kleinjung

Frank Kleinjung

Institut für Analytische Biochemie, Universität Potsdam, D-14943 Luckenwalde (Germany)

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Jennifer Ashurst

Jennifer Ashurst

Department of Plant Sciences, University of Cambridge, Downing St., Cambridge CB2 3EA (UK)

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Hartmut Oschkinat

Hartmut Oschkinat

Forschungsinstitut für Molekulare Pharmakologie, Alfred-Kowalke-Strasse 4, D-10315 Berlin (Germany)

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Rudolf Volkmer-Engert

Rudolf Volkmer-Engert

Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität Berlin, Schumannstrasse 20/21, D-10098 Berlin (Germany), Fax: (+49) 30-2802-6460

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Doris Koesling

Doris Koesling

Institut für Pharmakologie, Freie Universität Berlin, Thielallee 69–73, D-14195 Berlin (Germany)

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Jens Schneider-Mergener

Jens Schneider-Mergener

Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität Berlin, Schumannstrasse 20/21, D-10098 Berlin (Germany), Fax: (+49) 30-2802-6460

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Abstract

New interaction partners of a PDZ protein domain were identified through use of a library made up of all known human protein C-terminal peptides (“P” in the schematic representation). The library was displayed on a cellulose membrane by positional resolution of inverted peptides.

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