Volume 46, Issue 12 e14494

ORIGINAL ARTICLE

Cardiac hypertrophy and fibrosis were attenuated by olive leaf extract treatment in a rat model of diabetes

Ali Akbar Asghari,

Ali Akbar Asghari

Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

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Maryam Mahmoudabady,

Corresponding Author

Maryam Mahmoudabady

[email protected]

orcid.org/0000-0002-0466-1311

Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence

Maryam Mahmoudabady, Department of Physiology, Faculty of Medicine, Mashhad, Iran.

Email: [email protected]

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Zahra Mousavi Emadi,

Zahra Mousavi Emadi

Department of Pediatrics, Mashhad University of Medical Sciences, Mashhad, Iran

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Seyed Javad Hosseini,

Seyed Javad Hosseini

Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

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Hossein Salmani,

Hossein Salmani

Student Research Committee, Jiroft University of Medical Sciences, Jiroft, Iran

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Ali Akbar Asghari,

Ali Akbar Asghari

Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

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Maryam Mahmoudabady,

Corresponding Author

Maryam Mahmoudabady

[email protected]

orcid.org/0000-0002-0466-1311

Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence

Maryam Mahmoudabady, Department of Physiology, Faculty of Medicine, Mashhad, Iran.

Email: [email protected]

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Zahra Mousavi Emadi,

Zahra Mousavi Emadi

Department of Pediatrics, Mashhad University of Medical Sciences, Mashhad, Iran

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Seyed Javad Hosseini,

Seyed Javad Hosseini

Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

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Hossein Salmani,

Hossein Salmani

Student Research Committee, Jiroft University of Medical Sciences, Jiroft, Iran

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First published: 02 November 2022

https://doi.org/10.1111/jfbc.14494

Citations: 6

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Abstract

The key role of fibrosis and hypertrophy processes in developing diabetes-induced heart injury has been demonstrated. Considering the known hypoglycemic effects of olive leaf extract (OLE), we decided to investigate its potential effect and associated mechanisms on cardiac fibrosis and myocardial hypertrophy in streptozotocin (STZ)-induced diabetic rats. Eight groups were included in this study: control, diabetic, diabetic-OLEs (100, 200 and 400 mg/kg), diabetic-metformin (300 mg/kg), diabetic-valsartan (30 mg/kg), and diabetic-metformin/valsartan (300/30 mg/kg). After a treatment period of 6 weeks, echocardiography was used to assess cardiac function. Heart-to-body weight ratio (HW/BW) and fasting blood sugar (FBS) were measured. Myocardial histology was examined by Masson's trichrome staining. Gene expressions of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), β–myosin heavy chain (β-MHC), TGF-β1, TGF-β3, angiotensin II type 1 receptor (AT1), alpha-smooth muscle actin (α-SMA), and collagen were evaluated by the quantitative real-time PCR in heart tissue. A reduction in the FBS level and HW/BW ratio in the extract groups was obvious. The improvement of left ventricular dysfunction, cardiac myocytes hypertrophy, and myocardial interstitial fibrosis was also observed in treated groups. A lowering trend in the expression of all hypertrophic and fibrotic indicator genes was evident in the myocardium of OLE treated rats. Our data indicated that OLE could attenuate fibrosis and reduce myocardial hypertrophy markers, thus improving the cardiac function and structure in the STZ-induced diabetic rats.

Practical applications

This study demonstrates that olive leaf extract in addition to lowering blood glucose levels and the heart-to-body weight ratio (HW/BW) may also improve cardiac function and reduce cardiac hypertrophy and fibrosis in cardiac tissue, which leads to inhibition of diabetic heart damage. Thus it is possible that including olive leaf extracts in the diets of individuals with diabetes may assist in lowering cardiovascular disease risk factors.

CONFLICT OF INTEREST

There are no conflicts of interest declared by the authors.

Open Research

DATA AVAILABILITY STATEMENT

Data are available upon request.

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Cardiac hypertrophy and fibrosis were attenuated by olive leaf extract treatment in a rat model of diabetes

Abstract

Practical applications

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Cardiac hypertrophy and fibrosis were attenuated by olive leaf extract treatment in a rat model of diabetes

Abstract

Practical applications

CONFLICT OF INTEREST

Open Research

DATA AVAILABILITY STATEMENT

REFERENCES

Citing Literature

References

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