ORIGINAL ARTICLE
Systems pharmacology-based drug discovery and active mechanism of phlorotannins for type 2 diabetes mellitus by integrating network pharmacology and experimental evaluation
Jialiang Chen,
Ping Li,
Shuhong Ye,
Wei Li,
Ming Li,
Yan Ding,
Jialiang Chen
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
Search for more papers by this authorPing Li
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
Search for more papers by this authorShuhong Ye
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
Search for more papers by this authorWei Li
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu, South Korea
Search for more papers by this authorMing Li
College of Basic Medical Science, Dalian Medical University, Dalian, China
Search for more papers by this authorCorresponding Author
Yan Ding
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
Correspondence
Yan Ding, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
Email: [email protected]
Search for more papers by this authorJialiang Chen,
Ping Li,
Shuhong Ye,
Wei Li,
Ming Li,
Yan Ding,
Jialiang Chen
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
Search for more papers by this authorPing Li
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
Search for more papers by this authorShuhong Ye
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
Search for more papers by this authorWei Li
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu, South Korea
Search for more papers by this authorMing Li
College of Basic Medical Science, Dalian Medical University, Dalian, China
Search for more papers by this authorCorresponding Author
Yan Ding
School of Food Science and Technology, Dalian Polytechnic University, Dalian, China
Correspondence
Yan Ding, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
Email: [email protected]
Search for more papers by this authorAbstract
Phlorotannins, polyphenolic compounds that exist only in brown algae, have an effect on T2DM. However, the structure of phlorotannins is complex and diverse, and the complex role of therapeutic targets and active compounds has not been revealed. In this study, the potential targets and pharmacological effects of phlorotannins in the treatment of T2DM were identified based on network pharmacology and enzyme activity inhibition experiment. In total, 15 phlorotannins and 53 associated targets were yielded. Among them, SRC, ESR1, AKT1, HSP90AB1, and AR were defined as core targets. 527 GO biological processes items and 101 KEGG pathways were obtained, including EGFR tyrosine kinase inhibitor resistance, thyroid hormone signaling pathway, AGE-RAGE signaling pathway in diabetic complications, and VEGF signaling pathway. Phlorotannins could enable resistance against T2DM by inflammatory, survival, gene transcription, proliferation, apoptosis, and atherosclerosis. Finally, α-glucosidase inhibition assay and molecular docking proved the effect of selected phlorotannins on T2DM.
Practical applications
Phlorotannins are a kind of polyphenol compounds that only exists in brown algae. Its structure is polymerized by aromatic precursors phloroglucinol (1,3,5-trihydroxybenzene). They have aroused great interest due to their excellent and valuable biological activities. However, the structure of phlorotannins is complex and diverse, and the complex role of therapeutic targets and active compounds has not been revealed. In this study, the potential targets and pharmacological effects of phlorotannins in the treatment of T2DM were determined basis on network pharmacology and enzyme activity inhibition experiment. In conclusion, the results showed the value of phlorotannins treating on T2DM. Moreover, this study has great significance for improving the medicinal value of phlorotannins and screening natural products for the treatment of T2DM.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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