ERIODICTYOL DECREASES REACTIVE OXYGEN SPECIES PRODUCTION AND INHIBITS P47PHOX CLUSTERING AND TRANSLOCATION IN MONOCYTES
TZOU-CHI HUANG
Department of Food Science, National Pingtung University of Science and Technology, Pingtung, Taiwan
Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung, Taiwan
Search for more papers by this authorJEN-YU CHANG
Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung, Taiwan
Search for more papers by this authorHUNG-JEN LIU
Institute of Molecular Biology National Chung Hsing University, Taichung,Taiwan
Search for more papers by this authorSHINN-SHYONG TSAI
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan
Search for more papers by this authorTSUNG-CHOU CHANG
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan
Search for more papers by this authorCHI-TANG HO
Department of Food Science, Rutgers University, New Brunswick, NJ
Search for more papers by this authorCorresponding Author
KUO PIN CHUANG
Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 912, Pingtung, Taiwan
TEL: 886-8-7703202 ext 5333; FAX: 886-8-7700447; EMAIL: [email protected]Search for more papers by this authorTZOU-CHI HUANG
Department of Food Science, National Pingtung University of Science and Technology, Pingtung, Taiwan
Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung, Taiwan
Search for more papers by this authorJEN-YU CHANG
Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung, Taiwan
Search for more papers by this authorHUNG-JEN LIU
Institute of Molecular Biology National Chung Hsing University, Taichung,Taiwan
Search for more papers by this authorSHINN-SHYONG TSAI
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan
Search for more papers by this authorTSUNG-CHOU CHANG
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan
Search for more papers by this authorCHI-TANG HO
Department of Food Science, Rutgers University, New Brunswick, NJ
Search for more papers by this authorCorresponding Author
KUO PIN CHUANG
Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 912, Pingtung, Taiwan
TEL: 886-8-7703202 ext 5333; FAX: 886-8-7700447; EMAIL: [email protected]Search for more papers by this authorAbstract
ABSTRACT
Flavonoids have been proposed to have anti-inflammatory activity. Reactive oxygen species (ROS) are important mediators in the inflammatory response. We examined the effects of luteolin, quercetin and eriodictyol on ROS production in phorbol-12-myristate-13-acetate (PMA)-stimulated U937 cells. Eriodictyol inhibited ROS production at a lower inhibition concentration 50% (IC50) then luteolin and quercetin (eriodictyol : 0.822 µM, quercetin : 1.134 µM, luteolin : 1.026 µM). Moreover, eriodictyol inhibited ROS production in a dose- and time-dependent manner. Moreover, the p47phox subunit of nicotinamide adenine dinucleotide phosphate oxidase regulated PMA-mediated ROS production. After eriodictyol treatment, p47phox clustering and translocation also decreased on stimulation of U937 cells with PMA. This study suggests that eriodictyol governs immune responses by modulating p47phox clustering and translocation and ROS production in monocytes – evidence that eriodictyol is an immune regulator of ROS production during inflammatory responses.
PRACTICAL APPLICATIONS
This study suggests that eriodictyol governs immune responses by modulating p47phox clustering and translocation and ROS production in monocytes – evidence that eriodictyol is an immune regulator of ROS production during inflammatory responses.
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