Activity of mannose-binding lectin in centenarians
Rossella Tomaiuolo
CEINGE-Biotecnologie Avanzate scarl, Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy
Search for more papers by this authorAnna Ruocco
CEINGE-Biotecnologie Avanzate scarl, Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy
Search for more papers by this authorChiara Salapete
CEINGE-Biotecnologie Avanzate scarl, Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy
Search for more papers by this authorCiriaco Carru
Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy
Search for more papers by this authorClaudio Franceschi
Dipartimento di Patologia Sperimentale, Università di Bologna, Bologna, Italy
Search for more papers by this authorAngelo Zinellu
Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy
Search for more papers by this authorJames Vaupel
Max Planck Institute for Demographic Research, Rostock, Germany
Search for more papers by this authorChiara Bellia
Cattedra di Biochimica Clinica, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy
Search for more papers by this authorBruna Lo Sasso
Cattedra di Biochimica Clinica, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy
Search for more papers by this authorMarcello Ciaccio
Cattedra di Biochimica Clinica, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy
Search for more papers by this authorGiuseppe Castaldo
CEINGE-Biotecnologie Avanzate scarl, Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy
Search for more papers by this authorLuca Deiana
Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy
Azienda Ospedaliera Universitaria di Sassari, Sassari, Italy
Search for more papers by this authorRossella Tomaiuolo
CEINGE-Biotecnologie Avanzate scarl, Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy
Search for more papers by this authorAnna Ruocco
CEINGE-Biotecnologie Avanzate scarl, Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy
Search for more papers by this authorChiara Salapete
CEINGE-Biotecnologie Avanzate scarl, Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy
Search for more papers by this authorCiriaco Carru
Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy
Search for more papers by this authorClaudio Franceschi
Dipartimento di Patologia Sperimentale, Università di Bologna, Bologna, Italy
Search for more papers by this authorAngelo Zinellu
Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy
Search for more papers by this authorJames Vaupel
Max Planck Institute for Demographic Research, Rostock, Germany
Search for more papers by this authorChiara Bellia
Cattedra di Biochimica Clinica, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy
Search for more papers by this authorBruna Lo Sasso
Cattedra di Biochimica Clinica, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy
Search for more papers by this authorMarcello Ciaccio
Cattedra di Biochimica Clinica, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy
Search for more papers by this authorGiuseppe Castaldo
CEINGE-Biotecnologie Avanzate scarl, Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy
Search for more papers by this authorLuca Deiana
Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy
Azienda Ospedaliera Universitaria di Sassari, Sassari, Italy
Search for more papers by this authorSummary
We analyzed MBL2 gene variants in two cohorts of centenarians, octo-nonagenarians and nonagenarians, and in the general population, one from Sardinia Island (Italy), recruited in the frame of the AKea study, and another from Campania (southern Italy), to search for haplotypes related to longevity. We also assessed in vitro the effect of mannose-binding lectin (MBL) on various human cells at different stage of senescence. The frequency of high and null activity haplotypes was significantly lower, and the frequency of intermediate activity haplotype significantly higher in centenarians and in subjects between 80 and 99 years from both the cohorts as compared each to the general population from the same geographic area. Furthermore, serum MBL concentration (also after normalization to serum albumin) was significantly lower in centenarians and in octo- and nonagenarians as compared to the general population, suggesting that intermediate MBL haplotype/activity may be protective. We also demonstrated that in vitro MBL protein bound to senescent IMR90 fibroblasts thereby causing cell lysis, but not to other types of cycle-arrested cells not in senescence. This implicates a novel role of MBL in the clearance of senescent cells.
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