Immunogold labelling of tumour cells during NK/target cell interactions
Corresponding Author
M. J. ZANYK
Department of Pathology, The University of Western Ontario, London, Ontario, Canada and the Electron Microscopy Laboratory, St Joseph's Health Center, London, Ontario, Canada
Marien J. Zanyk, Department of Pathology, Immunobiology Laboratory, Rm 2–307, St Joseph's Health Center, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2.Search for more papers by this authorD. BANERJEE
Department of Pathology, The University of Western Ontario, London, Ontario, Canada and the Electron Microscopy Laboratory, St Joseph's Health Center, London, Ontario, Canada
Search for more papers by this authorS. A. HEARN
Department of Pathology, The University of Western Ontario, London, Ontario, Canada and the Electron Microscopy Laboratory, St Joseph's Health Center, London, Ontario, Canada
Search for more papers by this authorCorresponding Author
M. J. ZANYK
Department of Pathology, The University of Western Ontario, London, Ontario, Canada and the Electron Microscopy Laboratory, St Joseph's Health Center, London, Ontario, Canada
Marien J. Zanyk, Department of Pathology, Immunobiology Laboratory, Rm 2–307, St Joseph's Health Center, 268 Grosvenor Street, London, Ontario, Canada N6A 4V2.Search for more papers by this authorD. BANERJEE
Department of Pathology, The University of Western Ontario, London, Ontario, Canada and the Electron Microscopy Laboratory, St Joseph's Health Center, London, Ontario, Canada
Search for more papers by this authorS. A. HEARN
Department of Pathology, The University of Western Ontario, London, Ontario, Canada and the Electron Microscopy Laboratory, St Joseph's Health Center, London, Ontario, Canada
Search for more papers by this authorAbstract
Using a system of immunocolloidal gold labelling, we have monitored the expression and distribution of transferrin receptors (TfRs) within the K562 cell line, during NK/target cell interactions. An indirect method of immunolabelling was used to effectively immunolabel tumour cells without disrupting the natural effectontarget interactions. Successful localization of TfRs demonstrates the potential of the described technique for discerning antigenic distribution of other cell:cell interactions.
Immunolabelling has also provided a useful method for demonstrating receptor down-regulation within NK target cells, as a proposed cause of reduced receptor expression by TPA-treated cells. Following 30 and 60 min incubation periods with TPA, approximately 15 and 30%, respectively, of the gold/antibody complexes were relocated from the surface membrane to an intracellular location within endocytotic vesicles. The demonstration of receptor down-regulation is important as a proposed cause of TPA-induced tumour cell resistance to NK-mediated cytolysis.
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