Volume 79, Issue 2 pp. 281-293
ORIGINAL ARTICLE

Cattle dry manure fertilization increases forage yield of grass-legume mixtures, while maintaining the legume proportion and root-associated microbiota

Estefanía Oyharçabal

Corresponding Author

Estefanía Oyharçabal

Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Chubut (INTA EEA Chubut), Trelew, Argentina

Correspondence

Estefanía Oyharçabal, 25 de Mayo 4870 Trelew (CP 9100), Provincia del Chubut, Patagonia Argentina.

Email: [email protected]; [email protected]

Search for more papers by this author
Fernanda Covacevich

Fernanda Covacevich

Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC)/Fundación para las Investigaciones Biológicas aplicadas, Buenos Aires, Argentina

Search for more papers by this author
Ingrid Bain

Ingrid Bain

Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Chubut (INTA EEA Chubut), Trelew, Argentina

Search for more papers by this author
Claudina Soledad Acuña

Claudina Soledad Acuña

Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Chubut (INTA EEA Chubut), Trelew, Argentina

Search for more papers by this author
Germán Darío Berone

Germán Darío Berone

Facultad de Ciencias Agrarias–Universidad Nacional de Mar del Plata (FCA-UNMdP), Balcarce, Argentina

Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Balcarce (INTA EEA Balcarce), Trelew, Argentina

Search for more papers by this author
First published: 24 March 2024
Citations: 1

Abstract

The aim of this study was to find ways to improve the forage yield of grass-legume mixtures without compromising soil biodiversity. In Argentinean Patagonia, the effects of applying cattle dry manure (M) and urea (U) (0, 60, 120, or 240 kg N ha−1 year−1) were assessed on herbage production of irrigated fescue-lotus mixtures, as well as on the activity/abundance of autochthonous arbuscular mycorrhizal fungi and N-fixing rhizobium bacteria. We hypothesised that manure has advantages over urea in increasing forage yields while maintaining the proportion of legumes and root-associated microbiota. The 120 U, 240 U, and 240 M resulted in the greatest forage production; however, yield varied depending on the source applied. The high productivity of the 120 U and 240 U was probably due to the fast grass growth immediately after fertilization, which resulted in a depressed growth of the legume. The high yield of the swards fertilized with 240 M was probably due to slight and delayed growth of grass without legume yield decline. The highest radiation interception was found in swards with a low legume proportion, suggesting a light competition from grass fertilized with the highest urea doses, which were also consistent with the highest N and P nutritional status. The microbial activity/abundance were not affected by fertilization, but the final number of nodules was positively associated with the legume proportion. In conclusion, manure fertilization increased forage yield of the mixtures, while preserving the legume proportion and the root-associated microbiota. Our findings aid in reducing synthetic-N fertilizers applied in pasture-based livestock systems.

CONFLICT OF INTEREST STATEMENT

The authors have no conflicts of interest to disclose.

DATA AVAILABILITY STATEMENT

The data that support the findings of this study are available from the corresponding author upon reasonable request.

The full text of this article hosted at iucr.org is unavailable due to technical difficulties.