Volume 125, Issue 2 pp. 152-165
ORIGINAL ARTICLE

Fasudil alleviates brain damage in rats after carbon monoxide poisoning through regulating neurite outgrowth inhibitor/oligodendrocytemyelin glycoprotein signalling pathway

Li Wang

Li Wang

Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan Shandong, China

Department of Integration of Chinese and Western Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai Shandong, China

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Jianghua Xu

Jianghua Xu

Department of neurology, Yantai YEDA Hospital, Yantai Shandong, China

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Dadong Guo

Dadong Guo

Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan Shandong, China

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Xudong Zhou

Xudong Zhou

The First Affiliated Hospital of Shandong, University of Traditional Chinese Medicine, Jinan Shandong, China

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Wenwen Jiang

Wenwen Jiang

Department of Integration of Chinese and Western Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai Shandong, China

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Jinglin Wang

Jinglin Wang

Emergency Centre, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai Shandong, China

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Jiyou Tang

Jiyou Tang

Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan Shandong, China

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Yong Zou

Yong Zou

Department of Integration of Chinese and Western Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai Shandong, China

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Mingjun Bi

Corresponding Author

Mingjun Bi

Emergency Centre, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai Shandong, China

Correspondence

Qin Li and Mingjun Bi, Department of Integration of Chinese and Western Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai Shandong, China.

Emails: [email protected]; [email protected]

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Qin Li

Corresponding Author

Qin Li

Department of Integration of Chinese and Western Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai Shandong, China

Correspondence

Qin Li and Mingjun Bi, Department of Integration of Chinese and Western Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai Shandong, China.

Emails: [email protected]; [email protected]

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First published: 27 March 2019
Citations: 8
Wang and Xu contributed equally as co-first authors.

Abstract

Carbon monoxide (CO) poisoning can lead to many serious neurological symptoms. Currently, there are no effective therapies for CO poisoning. In this study, rats exposed to CO received hyperbaric oxygen therapy, and those in the Fasudil group were given additional Fasudil injection once daily. We found that the escape latency in CO poisoning group (CO group) was significantly prolonged, the T1/Ttotal was obviously decreased, and the mean escape time and the active escape latency were notably extended compared with those in normal control group (NC group, P < 0.05). After administration of Fasudil, the escape latency was significantly shortened, T1/Ttotal was gradually increased as compared with CO group (>1 week, P < 0.05). Ultrastructural damage of neurons and blood-brain barrier of rats was serious in CO group, while the structural and functional integrity of neuron and mitochondria maintained relatively well in Fasudil group. Moreover, we also noted that the expressions of neurite outgrowth inhibitor (Nogo), oligodendrocyte-myelin glycoprotein (OMgp) and Rock in brain tissue were significantly increased in CO group, and the elevated levels of the three proteins were still observed at 2 months after CO poisoning. Fasudil markedly reduced their expressions compared with those of CO group (P < 0.05). In summary, the activation of Nogo-OMgp/Rho signalling pathway is associated with brain injury in rats with CO poisoning. Fasudil can efficiently down-regulate the expressions of Nogo, OMgp and Rock proteins, paving a way for the treatment of acute brain damage after CO poisoning.

CONFLICT OF INTEREST

The authors declare that they have no competing financial interests.

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