American Journal of Reproductive Immunology

Volume 78, Issue 1 e12665

ORIGINAL ARTICLE

Changes in expression of the CD200 tolerance-signaling molecule and its receptor (CD200R) by villus trophoblasts during first trimester missed abortion and in chronic histiocytic intervillositis

David A. Clark,

Corresponding Author

David A. Clark

[email protected]

orcid.org/0000-0002-8032-1028

Department of Medicine, McMaster University, Hamilton, Ontario, Canada

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

Correspondence

David A. Clark, McMaster University, Hamilton, Ontario, Canada.

Email: [email protected]

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Jennifer M. Dmetrichuk,

Jennifer M. Dmetrichuk

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

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Elizabeth McCready,

Elizabeth McCready

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

Genetics Services, Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, Ontario, Canada

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Sukhbinder Dhesy-Thind,

Sukhbinder Dhesy-Thind

Department of Oncology, McMaster University, Hamilton, Ontario, Canada

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Jorge L. Arredondo,

Jorge L. Arredondo

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

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David A. Clark,

Corresponding Author

David A. Clark

[email protected]

orcid.org/0000-0002-8032-1028

Department of Medicine, McMaster University, Hamilton, Ontario, Canada

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

Correspondence

David A. Clark, McMaster University, Hamilton, Ontario, Canada.

Email: [email protected]

Search for more papers by this author

Jennifer M. Dmetrichuk,

Jennifer M. Dmetrichuk

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

Search for more papers by this author

Elizabeth McCready,

Elizabeth McCready

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

Genetics Services, Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, Ontario, Canada

Search for more papers by this author

Sukhbinder Dhesy-Thind,

Sukhbinder Dhesy-Thind

Department of Oncology, McMaster University, Hamilton, Ontario, Canada

Search for more papers by this author

Jorge L. Arredondo,

Jorge L. Arredondo

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada

Search for more papers by this author

First published: 22 March 2017

https://doi.org/10.1111/aji.12665

Citations: 16

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Abstract

Problem

Expression of CD200 at the feto-maternal interface is associated with successful murine and human pregnancy. CD200 binding to CD200 receptors on lymphomyeloid cells suppresses inflammation and induces Tregs. CD200 receptors are also expressed on mouse and human placental trophoblast cells. What is the expression of CD200 and CD200R in human missed abortions which have preserved Treg levels and in chronic histiocytic intervillositis (CHI) where maternal inflammatory cells cause IUGR?

Methods

Immunohistiochemistry for CD200, CD200R, and Ki67 using human placental sections from missed abortions, term placenta, and CHI. PCR testing was done for trisomy in missed abortion.

Results

CD200 and CD200R were expressed by human villus trophoblasts from 2 weeks post-implantation to term. Cytotrophoblast proliferation (Ki-67⁺ count) decreased at term. In first trimester missed abortion cases, CD200>CD200R villus trophoblasts accompanied missed abortion of non-trisomic male fetuses. CD200 and Ki67⁺ trophoblast proliferation was preserved in CHI with maternal inflammatory cell infiltration but CD200R was greatly decreased.

Conclusion

Residual CD200 activity may prevent completion of abortions via induction of Treg cells. In CHI, infiltrating maternal effector T cells may block Treg induction. An autocrine role for CD200-CD200R interaction versus inhibition of soluble CD200 by soluble CD200R is discussed.

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Volume78, Issue1

July 2017

e12665

Changes in expression of the CD200 tolerance-signaling molecule and its receptor (CD200R) by villus trophoblasts during first trimester missed abortion and in chronic histiocytic intervillositis

Abstract

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Methods

Results

Conclusion

References

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Changes in expression of the CD200 tolerance-signaling molecule and its receptor (CD200R) by villus trophoblasts during first trimester missed abortion and in chronic histiocytic intervillositis

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Methods

Results

Conclusion

References

Citing Literature

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