Transcriptome analysis of the response of Lactiplantibacillus plantarum IMAU10120 to infection by phage P2
Qiannan Wen
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation, Validation, Visualization, Writing - review & editing
Search for more papers by this authorRuirui Lv
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation, Visualization
Search for more papers by this authorCan Zhang
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation
Search for more papers by this authorWeiqi Lian
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation
Search for more papers by this authorRunze Liu
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation
Search for more papers by this authorCorresponding Author
Xia Chen
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Author for correspondence. E-mail: [email protected]
Contribution: Funding acquisition, Supervision, Writing - review & editing
Search for more papers by this authorQiannan Wen
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation, Validation, Visualization, Writing - review & editing
Search for more papers by this authorRuirui Lv
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation, Visualization
Search for more papers by this authorCan Zhang
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation
Search for more papers by this authorWeiqi Lian
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation
Search for more papers by this authorRunze Liu
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Contribution: Investigation
Search for more papers by this authorCorresponding Author
Xia Chen
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018 China
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018 China
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018 China
Author for correspondence. E-mail: [email protected]
Contribution: Funding acquisition, Supervision, Writing - review & editing
Search for more papers by this authorAbstract
Bacteriophage infection of lactic acid bacteria remains a significant problem in the food fermentation industry. To improve understanding about the underlying mechanism of phage–host infection, we used RNA sequencing technology to investigate the effect of phage infection on the expression of host genes based on the one-step growth curve. Results identified a total of 3207 genes, of which 178 showed a significant differential expression in phage-infected host. Moreover, bioinformatic analyses unravelled that these genes regulated many processes in host bacteria, including ribosome, RNA polymerase, DNA replication and metabolic pathways. This study further elucidates the response of host bacteria to phage infection. These results provide valuable data for developing effective phage control measures and improving the success rate of fermentation in the dairy industry.
CONFLICT OF INTEREST STATEMENT
The authors declare no conflict of interest in this study.
Open Research
DATA AVAILABILITY STATEMENT
The transcriptome sequencing of phage P2 has been uploaded to the SRA database of the National Center for Biotechnology Information (NCBI), under the accession number SRP419172.
Supporting Information
| Filename | Description |
|---|---|
| idt13055-sup-0001-TableS1.docxWord 2007 document , 24.1 KB | Table S1 Primer pairs designed by primer 5 software. |
| idt13055-sup-0002-TableS2.docxWord 2007 document , 42.7 KB | Table S2 Comparison of sequencing data from each sample before and after filtration. |
| idt13055-sup-0003-TableS3.docxWord 2007 document , 18.1 KB | Table S3 Percentage of reads mapping to the host and phage genome. |
| idt13055-sup-0004-TableS4.docxWord 2007 document , 28.6 KB | Table S4 Functional annotation of genes in the phage P2 genome. |
| idt13055-sup-0005-TableS5.docxWord 2007 document , 68.8 KB | Table S5 DEGs involved in pathways in response to phage infection at different time points. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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