Whey protein-derived peptides improve blood profile and enhance muscular endurance in mice
Da-Min Jung
Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon, 34134 South Korea
Contribution: Conceptualization, Investigation, Validation, Data curation, Writing - original draft
Search for more papers by this authorWoo-Jin Ki
Division of Animal Resource Science, Chungnam National University, Daejeon, 34134 South Korea
Contribution: Data curation, Investigation, Formal analysis, Visualization
Search for more papers by this authorYoung-Soon Lim
Department of Animal Biotechnology, Sangji University, Wonju, 26339 South Korea
Contribution: Resources
Search for more papers by this authorCorresponding Author
Myoung-Soo Nam
Division of Animal Resource Science, Chungnam National University, Daejeon, 34134 South Korea
Author for correspondence. E-mail: [email protected]; [email protected]
Contribution: Project administration, Conceptualization, Funding acquisition
Search for more papers by this authorCorresponding Author
Kee K. Kim
Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon, 34134 South Korea
Author for correspondence. E-mail: [email protected]; [email protected]
Contribution: Conceptualization, Funding acquisition, Writing - original draft, Writing - review & editing, Supervision, Resources, Methodology
Search for more papers by this authorDa-Min Jung
Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon, 34134 South Korea
Contribution: Conceptualization, Investigation, Validation, Data curation, Writing - original draft
Search for more papers by this authorWoo-Jin Ki
Division of Animal Resource Science, Chungnam National University, Daejeon, 34134 South Korea
Contribution: Data curation, Investigation, Formal analysis, Visualization
Search for more papers by this authorYoung-Soon Lim
Department of Animal Biotechnology, Sangji University, Wonju, 26339 South Korea
Contribution: Resources
Search for more papers by this authorCorresponding Author
Myoung-Soo Nam
Division of Animal Resource Science, Chungnam National University, Daejeon, 34134 South Korea
Author for correspondence. E-mail: [email protected]; [email protected]
Contribution: Project administration, Conceptualization, Funding acquisition
Search for more papers by this authorCorresponding Author
Kee K. Kim
Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon, 34134 South Korea
Author for correspondence. E-mail: [email protected]; [email protected]
Contribution: Conceptualization, Funding acquisition, Writing - original draft, Writing - review & editing, Supervision, Resources, Methodology
Search for more papers by this authorAbstract
Whey protein-derived peptides (WPP) can exert diverse physiological activities. However, their effects on exercise ability remain largely unknown. Herein, we investigated the effects of WPP on various physiological activities in cells, as well as on the blood profile and exercise ability of mice, aiming to develop bioactive peptides for enhancing muscular endurance. The intake of WPP induced a positive blood profile by lowering total cholesterol and significantly enhanced the muscular endurance of mice in treadmill endurance tests. Collectively, our results suggest the usefulness of WPP as agents for inducing vascular health, contributing to exercise ability by enhancing muscular endurance.
CONFLICT OF INTEREST
All authors declare no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| idt13038-sup-0001-TableS1.docxWord 2007 document , 16.8 KB | Table S1. Primer information for qRT-PCR. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
REFERENCES
- Ballantyne C M, Olsson A G, Cook T J, Mercuri M F, Pedersen T R and Kjekshus J (2001) Influence of low high-density lipoprotein cholesterol and elevated triglyceride on coronary heart disease events and response to simvastatin therapy in 4S. Circulation 104 3046–3051.
- Bian Y, Wei J, Zhao C and Li G (2020) Natural polyphenols targeting senescence: A novel prevention and therapy strategy for cancer. International Journal of Molecular Sciences 21 1–17.
- Butcher J T, Mintz J D, Larion S, Qiu S, Ruan L, Fulton D J and Stepp D W (2018) Increased muscle mass protects against hypertension and renal injury in obesity. Journal of the American Heart Association 7 e009358.
- Chen J, Yang J, Ma L, Li J, Shahzad N and Kim C K (2020) Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids. Scientific Reports 102 611.
- Ciesielska A, Matyjek M and Kwiatkowska K (2021) TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling. Cellular and Molecular Life Sciences 781 233–1261.
- Cristofalo V J (2005) SA beta Gal staining: biomarker or delusion. Experimental Gerontology 40 836–838.
- Csekes E and Rackova L (2021) Skin aging, cellular senescence and natural polyphenols. International Journal of Molecular Sciences 22 12641.
- El-Zayat S R, Sibaii H and Mannaa F A (2019) Toll-like receptors activation, signaling, and targeting: An overview. Bulletin of the National Research Centre 43 1157023.
10.1186/s42269-019-0227-2 Google Scholar
- Grażyna C, Hanna C, Adam A and Magdalena B M (2017) Natural antioxidants in milk and dairy products. International Journal of Dairy Technology 70 165–178.
- Higashi Y (2023) Endothelial function in dyslipidemia: Roles of LDL-cholesterol, HDL-cholesterol and triglycerides. Cells 12 1293.
- Huang Y, Ruan G, Qin Z, Li H and Zheng Y (2017) Antioxidant activity measurement and potential antioxidant peptides exploration from hydrolysates of novel continuous microwave-assisted enzymolysis of the Scomberomorus niphonius protein. Food Chemistry 223 89–95.
- Jeong E W, Park G R, Kim J, Yun S Y, Imm J Y and Lee H G (2021) Effect of modified casein to whey protein ratio on dispersion stability, protein quality and body composition in rats. Food Sci Anim Resour 41 855–868.
- Jung H, Jung D, Lee J, Ki W, Lee J M, Kim E M, Nam M S and Kim K K (2022) Bioactive peptides in the pancreatin-hydrolysates of whey protein support cell proliferation and scavenge reactive oxygen species. Animal Cells and Systems (Seoul) 26 232–242.
- Khan I T, Nadeem M, Imran M, Ullah R, Ajmal M and Jaspal M H (2019) Antioxidant properties of Milk and dairy products: A comprehensive review of the current knowledge. Lipids in Health and Disease 18 41.
- Kong S, Choi H R, Kim Y J, Lee Y S, Park K C and Kwak S Y (2020) Milk protein-derived antioxidant Tetrapeptides as potential Hypopigmenting agents. Antioxidants (Basel) 9 1106.
- Koopman R, Crombach N, Gijsen A P, Walrand S, Fauquant J, Kies A K, Lemosquet S, Saris W H M, Boirie Y and van Loon L J C (2009) Ingestion of a protein hydrolysate is accompanied by an accelerated in vivo digestion and absorption rate when compared with its intact protein. The American Journal of Clinical Nutrition 90 106–115.
- Lee B Y, Han J A, Im J S, Morrone A, Johung K, Goodwin E C, Kleijer W J, DiMaio D and Hwang E S (2006) Senescence-associated beta-galactosidase is lysosomal beta-galactosidase. Aging Cell 5 5187–5195.
- Leslie W D, Schousboe J T, Morin S N, Martineau P, Lix L M, Johansson H, McCloskey E V, Harvey N C and Kanis J A (2020) Loss in DXA-estimated total body lean mass but not fat mass predicts incident major osteoporotic fracture and hip fracture independently from FRAX: A registry-based cohort study. Archives of Osteoporosis 15 96.
- Liao N, Shi Y, Zhang C, Zheng Y, Wang Y, Zhao B, Zeng Y, Liu X and Liu J (2019) Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion. Stem Cell Research & Therapy 10 306.
- Madureira A R, Tavares T, Gomes A M, Pintado M E and Malcata F X (2010) Invited review: Physiological properties of bioactive peptides obtained from whey proteins. Journal of Dairy Science 93 437–455.
- Maldonado Galdeano C, Novotny Nunez I, De Moreno De Leblanc A, Carmuega E, Weill R and Perdigon G (2011) Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice. BMC Gastroenterology 11 64.
- Markowiak P and Slizewska K (2017) Effects of probiotics, prebiotics, and Synbiotics on human health. Nutrients 9 572754.
- Moro, T, Brightwell, C R, Velarde, B, Fry C. S., Nakayama K., Sanbongi C., Volpi E., Rasmussen B. B. (2019) Whey protein hydrolysate increases amino acid uptake, mTORC1 signaling, and protein synthesis in skeletal muscle of healthy young men in a randomized crossover trial. The Journal of Nutrition, 49, 1149–1158.
10.1093/jn/nxz053 Google Scholar
- Ormstad H, Verkerk R, Aass H C, Amthor K F and Sandvik L (2013) Inflammation-induced catabolism of tryptophan and tyrosine in acute ischemic stroke. Journal of Molecular Neuroscience 51 893–902.
- Pezeshki A, Fahim A and Chelikani P K (2015) Dietary whey and casein differentially affect energy balance, gut hormones, glucose metabolism, and taste preference in diet-induced obese rats. The Journal of Nutrition 145 2236–2244.
- Ren J L, Pan J S, Lu Y P, Sun P and Han J (2009) Inflammatory signaling and cellular senescence. Cellular Signalling 21 378–383.
- Rocile A F (1994) Sarcopenia: A critical review of its measurements and health-related significance in the middle-aged and elderly. American Journal of Human Biology 6 33–42.
- Roldano Scognamiglio A A, Negut C, Piccolotto R, de Kreutzenberg S V and Tiengo A (2004) The effects of oral amino acid intake on ambulatory capacity in elderly subjects. Aging Clinical and Experimental Research 16 443–447.
- Rutherfurd-Markwick K J (2012) Food proteins as a source of bioactive peptides with diverse functions. The British Journal of Nutrition 108(Suppl 2) S149–S157.
- Sorgdrager F J H, Naude P J W, Kema I P, Nollen E A and Deyn P P (2019) Tryptophan metabolism in Inflammaging: From biomarker to therapeutic target. Frontiers in Immunology 10 2565.
- Stelwagen K, Carpenter E, Haigh B, Hodgkinson A and Wheeler T T (2009) Immune components of bovine colostrum and milk. Journal of Animal Science 87 3–9.
- Stobiecka M, Krol J and Brodziak A (2022) Antioxidant activity of Milk and dairy products. Animals (Basel) 12 245.
- Swanson L, Katkar G D, Tam J et al. (2020) TLR4 signaling and macrophage inflammatory responses are dampened by GIV/Girdin. Proceedings of the National Academy of Sciences of the United States of America 117 26895–26906.
- Szydlowska A and Sionek B (2022) Probiotics and Postbiotics as the functional food components affecting the immune response. Microorganisms 11 104.
- Takaeko Y, Kajikawa M, Kishimoto S et al. (2020) Low levels of low-density lipoprotein cholesterol and endothelial function in subjects without lipid-lowering therapy. Journal of Clinical Medicine 9 3796.
- Varesi A, Chirumbolo S, Campagnoli L I M, Pierella E, Piccini G B, Carrara A, Ricevuti G, Scassellati C, Bonvicini C and Pascale A (2022) The role of antioxidants in the interplay between oxidative stress and senescence. Antioxidants (Basel) 11 1224.
- Yin K, Ismail A, Mohamed M and Kwei Y (2019) Euphorbia hirta methanolic extract displays potential antioxidant activity for the development of local natural products. Pharmacognosy Research 11 78.
