MiR-96 regulates bone metabolism by targeting osterix
Hua Liu
Department of Orthopedics, The Xiangya Hospital of Central South University, Changsha, China
Search for more papers by this authorQing Liu
Department of Orthopedics, The Xiangya Hospital of Central South University, Changsha, China
Search for more papers by this authorXian-Ping Wu
Institute of Endocrinology & Metabolism, The Second Xiangya Hospital of Central South University, Changsha, China
Search for more papers by this authorHong-Bo He
Department of Orthopedics, The Xiangya Hospital of Central South University, Changsha, China
Search for more papers by this authorCorresponding Author
Lei Fu
Department of Infectious Diseases, The Xiangya Hospital of Central South University, Changsha, China
Correspondence
Lei Fu, Department of Infectious Diseases, The Xiangya Hospital of Central South University, Changsha, China.
Email: [email protected]
Search for more papers by this authorHua Liu
Department of Orthopedics, The Xiangya Hospital of Central South University, Changsha, China
Search for more papers by this authorQing Liu
Department of Orthopedics, The Xiangya Hospital of Central South University, Changsha, China
Search for more papers by this authorXian-Ping Wu
Institute of Endocrinology & Metabolism, The Second Xiangya Hospital of Central South University, Changsha, China
Search for more papers by this authorHong-Bo He
Department of Orthopedics, The Xiangya Hospital of Central South University, Changsha, China
Search for more papers by this authorCorresponding Author
Lei Fu
Department of Infectious Diseases, The Xiangya Hospital of Central South University, Changsha, China
Correspondence
Lei Fu, Department of Infectious Diseases, The Xiangya Hospital of Central South University, Changsha, China.
Email: [email protected]
Search for more papers by this authorSummary
MicroRNAs (miRNAs) play important roles in bone metabolism and aging. Here we show that miR-96 was markedly up-regulated in serum of elderly patients with osteoporosis by miRNA microarray analysis and qRT-PCR. Moreover miR-96 was also up-regulated in bone marrow mesenchymal stem cells (BMSCs) of aged humans and mice. Our results show that the over-expression of miR-96 reduced osteogenic differentiation of BMSCs, whereas the inhibition of miR-96 increased osteogenic differentiation of BMSCs. At the molecular level, miR-96 regulated osteogenesis by targeting osterix. Interestingly, over-expression of miR-96 in young mice by intravenous injection of agomiR-96 developed a low bone mass due to impaired osteogenesis. However, inhibition of miR-96 in aged mice attenuated the age-related bone loss. Thus, our data suggest that miR-96 regulates osteogenesis and may represent a potential diagnostic marker or therapeutic target for age-related bone loss.
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