Design, construction, and biological testing of an implantable porous trilayer scaffold for repairing osteoarthritic cartilage
Yaima Campos
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Biomaterials Center, University of Havana, Havana, Cuba
Search for more papers by this authorFrancisco J. Sola
Biomaterials Center, University of Havana, Havana, Cuba
Search for more papers by this authorAmisel Almirall
Biomaterials Center, University of Havana, Havana, Cuba
Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan
Search for more papers by this authorGastón Fuentes
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Biomaterials Center, University of Havana, Havana, Cuba
Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan
Bioforge Lab, Campus Miguel Delibes, CIBER-BBN, Universidad de Valladolid, Edificio LUCIA, Valladolid, Spain
Search for more papers by this authorChristina Eich
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Search for more papers by this authorIvo Que
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Search for more papers by this authorEric Kaijzel
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Search for more papers by this authorYasuhiko Tabata
Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan
Search for more papers by this authorLuis Quintanilla
Bioforge Lab, Campus Miguel Delibes, CIBER-BBN, Universidad de Valladolid, Edificio LUCIA, Valladolid, Spain
Search for more papers by this authorJosé C. Rodríguez-Cabello
Bioforge Lab, Campus Miguel Delibes, CIBER-BBN, Universidad de Valladolid, Edificio LUCIA, Valladolid, Spain
Search for more papers by this authorCorresponding Author
Luis J. Cruz
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Correspondence
Luis J. Cruz, Translational Nanomedicine and Imaging Group, Department of Radiology, C2-S-room 187, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
Email: [email protected]
Search for more papers by this authorYaima Campos
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Biomaterials Center, University of Havana, Havana, Cuba
Search for more papers by this authorFrancisco J. Sola
Biomaterials Center, University of Havana, Havana, Cuba
Search for more papers by this authorAmisel Almirall
Biomaterials Center, University of Havana, Havana, Cuba
Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan
Search for more papers by this authorGastón Fuentes
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Biomaterials Center, University of Havana, Havana, Cuba
Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan
Bioforge Lab, Campus Miguel Delibes, CIBER-BBN, Universidad de Valladolid, Edificio LUCIA, Valladolid, Spain
Search for more papers by this authorChristina Eich
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Search for more papers by this authorIvo Que
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Search for more papers by this authorEric Kaijzel
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Search for more papers by this authorYasuhiko Tabata
Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan
Search for more papers by this authorLuis Quintanilla
Bioforge Lab, Campus Miguel Delibes, CIBER-BBN, Universidad de Valladolid, Edificio LUCIA, Valladolid, Spain
Search for more papers by this authorJosé C. Rodríguez-Cabello
Bioforge Lab, Campus Miguel Delibes, CIBER-BBN, Universidad de Valladolid, Edificio LUCIA, Valladolid, Spain
Search for more papers by this authorCorresponding Author
Luis J. Cruz
Translational Nanobiomaterials and Imaging, Department of Radiology, Leiden University Medical Centre, Leiden, The Netherlands
Correspondence
Luis J. Cruz, Translational Nanomedicine and Imaging Group, Department of Radiology, C2-S-room 187, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
Email: [email protected]
Search for more papers by this authorAbstract
Various tissue engineering systems for cartilage repair have been designed and tested over the past two decades, leading to the development of many promising cartilage grafts. However, no one has yet succeeded in devising an optimal system to restore damaged articular cartilage. Here, the design, assembly, and biological testing of a porous, chitosan/collagen-based scaffold as an implant to repair damaged articular cartilage is reported. Its gradient composition and trilayer structure mimic variations in natural cartilage tissue. One of its layers includes hydroxyapatite, a bioactive component that facilitates the integration of growing tissue on local bone in the target area after scaffold implantation. The scaffold was evaluated for surface morphology; rheological performance (storage, loss, complex, and time-relaxation moduli at 1 kHz); physiological stability; in vitro activity and cytotoxicity (on a human chondrocyte C28 cell line); and in vivo performance (tissue growth and biodegradability), in a murine model of osteoarthritis. The scaffold was shown to be mechanically resistant and noncytotoxic, favored tissue growth in vivo, and remained stable for 35 days postimplantation in mice. These encouraging results highlight the potential of this porous chitosan/collagen scaffold for clinical applications in cartilage tissue engineering.
CONFLICT OF INTEREST
There are no conflicts to declare.
Supporting Information
| Filename | Description |
|---|---|
| term3001-sup-0001-Supplementary.docxWord 2007 document , 2.8 MB |
Figure S1. Preparation of the trilayer scaffold. Figure S2. Micro-CT of scaffold specimens in different planes for better view of hydroxyapatite localizations |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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