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Solution structure of the DNA-binding domain of the heat shock transcription factor determined by multidimensional heteronuclear magnetic resonance spectroscopy
Fred F. Damberger,
Jeffrey G. Pelton,
Celia J. Harrison,
Hillary C.M. Nelson,
David E. Wemmer,
Fred F. Damberger
Biophysics Graduate Group, University of California, Berkeley, California 94720
Search for more papers by this authorJeffrey G. Pelton
Structural Biology Division of Lawrence Berkeley Laboratory, 1 Cyclotron Road, Berkeley, California 94720
Search for more papers by this authorCelia J. Harrison
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720
Search for more papers by this authorHillary C.M. Nelson
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720
Search for more papers by this authorCorresponding Author
David E. Wemmer
Department of Chemistry, University of California, Berkeley, California 94720
Department of Chemistry, University of California, Berkeley, California 94720Search for more papers by this authorFred F. Damberger,
Jeffrey G. Pelton,
Celia J. Harrison,
Hillary C.M. Nelson,
David E. Wemmer,
Fred F. Damberger
Biophysics Graduate Group, University of California, Berkeley, California 94720
Search for more papers by this authorJeffrey G. Pelton
Structural Biology Division of Lawrence Berkeley Laboratory, 1 Cyclotron Road, Berkeley, California 94720
Search for more papers by this authorCelia J. Harrison
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720
Search for more papers by this authorHillary C.M. Nelson
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720
Search for more papers by this authorCorresponding Author
David E. Wemmer
Department of Chemistry, University of California, Berkeley, California 94720
Department of Chemistry, University of California, Berkeley, California 94720Search for more papers by this authorAbstract
The solution structure of the 92-residue DNA-binding domain of the heat shock transcription factor from Kluyveromyces lactis has been determined using multidimensional NMR methods. Three-dimensional (3D) triple resonance, 1H-13C-13C-1H total correlation spectroscopy, and 15N-separated total correlation spectroscopy-heteronuclear multiple quantum correlation experiments were used along with various 2D spectra to make nearly complete assignments for the backbone and side-chain 1H, 15N, and 13C resonances. Five-hundred eighty-three NOE constraints identified in 3D 13C- and l5N-separated NOE spectroscopy (NOESY)-heteronuclear multiple quantum correlation spectra and a 4-dimensional 13C/13C-edited NOESY spectrum, along with 35 ø, 9 χ1, and 30 hydrogen bond constraints, were used to calculate 30 structures by a hybrid distance geometry/simulated annealing protocol, of which 24 were used for structural comparison. The calculations revealed that a 3-helix bundle packs against a small 4-stranded antiparallel β-sheet. The backbone RMS deviation (RMSD) for the family of structures was 1.03 ± 0.19 Å with respect to the average structure. The topology is analogous to that of the C-terminal domain of the catabolite gene activator protein and appears to be in the helix-turn-helix family of DNA-binding proteins. The overall fold determined by the NMR data is consistent with recent crystallographic work on this domain (Harrison CJ, Bohm AA, Nelson HCM, 1994, Science 263:224) as evidenced by RMSD between backbone atoms in the NMR and X-ray structures of 1.77 ± 0.20 Å. Several differences were identified some of which may be due to protein-protein interactions in the crystal.
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