Synthesis and Incorporation of Quaternary Ammonium Silane Antimicrobial into Self-Crosslinked Type I Collagen Scaffold: A Hybrid Formulation for 3D Printing
Ranjeet Ajit Bapat
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorSenthil Kumar Muthusamy
Oral Sciences, Faculty of Dentistry, National University of Singapore, 21 Lower Kent Ridge Rd, Singapore, 119077 Singapore
Search for more papers by this authorPreena Sidhu
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorKit-Kay Mak
School of Pharmacy, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorAbhishek Parolia
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorMallikarjuna Rao Pichika
School of Pharmacy, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorLiang Lin Seow
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorCorresponding Author
Cao Tong
Oral Sciences, Faculty of Dentistry, National University of Singapore, 21 Lower Kent Ridge Rd, Singapore, 119077 Singapore
E-mail: [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Umer Daood
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
E-mail: [email protected]; [email protected]
Search for more papers by this authorRanjeet Ajit Bapat
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorSenthil Kumar Muthusamy
Oral Sciences, Faculty of Dentistry, National University of Singapore, 21 Lower Kent Ridge Rd, Singapore, 119077 Singapore
Search for more papers by this authorPreena Sidhu
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorKit-Kay Mak
School of Pharmacy, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorAbhishek Parolia
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorMallikarjuna Rao Pichika
School of Pharmacy, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorLiang Lin Seow
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
Search for more papers by this authorCorresponding Author
Cao Tong
Oral Sciences, Faculty of Dentistry, National University of Singapore, 21 Lower Kent Ridge Rd, Singapore, 119077 Singapore
E-mail: [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Umer Daood
Division of Restorative Dentistry, School of Dentistry, International Medical University Kuala Lumpur, 126, Jalan Jalil Perkasa 19, Bukit Jalil, 57000 Bukit Jalil, Wilayah Persekutuan, Kuala Lumpur, Malaysia
E-mail: [email protected]; [email protected]
Search for more papers by this authorAbstract
Novel 3D-biomaterial scaffold is constructed having a combination of a new quaternary ammonium silane (k21) antimicrobial impregnated in 3D collagen printed scaffolds cross linked with Riboflavin in presence of d-alpha-tocopheryl poly(ethyleneglycol)-1000-succinate. Groups of “0.1% and 0.2% k21”, and “0.1% and 0.2% Chlorhexidine (CHX)” are prepared. k21/CHX with neutralized collagen is printed with BioX. Riboflavin is photo-activated and examined using epifluorescence for Aggregatibacter actinomycetemcomitans (7-days). Collagen is examined using TEM and measured for porosity, and shape-fitting. Raman and tandem mass/solid-state are performed with molecular-docking and circular-dichroism. X-ray diffractions, rheological tests, contact angle, and ninhydrin assay are conducted. k21 samples demonstrated collagen aggregates while 0.1% CHX and 0.2% CHX showed irregularities. Porosity of control and “0.1% and 0.2% k21” scaffolds show no differences. Low contact angle, improved elastic-modulus, rigidity, and smaller strain in k21 groups are seen. Bacteria are reduced and strong organic intensities are seen in k21 scaffolds. Simulation shows hydrophobicity/electrostatic interaction. Crosslinking is observed in 0.2% CHX/79% and 0.2% k21/80%. Circular dichroism for k21 are suggestive of triple helix. XRD patterns appear at d = 5.97, 3.03, 2.78, 2.1, and 2.90 A°. 3D-printing of collagen impregnated with quaternary ammonium silane produces a promising scaffold with antimicrobial potency and structural stability.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.
Supporting Information
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| mabi202100326-sup-0001-SuppMat.MP47 MB | Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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