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Amphiphilic PEO-b-PBLG Diblock and PBLG-b-PEO-b-PBLG Triblock Copolymer Based Nanoparticles: Doxorubicin Loading and In Vitro Evaluation

Dipti Kakkar

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054 India

Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, 110016 India

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Silvia Mazzaferro,

Silvia Mazzaferro

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Julie Thevenot,

Julie Thevenot

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Christophe Schatz,

Christophe Schatz

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Anant Bhatt,

Anant Bhatt

Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054 India

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Bilikere S. Dwarakanath,

Bilikere S. Dwarakanath

Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054 India

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Harpal Singh,

Harpal Singh

Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, 110016 India

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Anil K. Mishra,

Corresponding Author

Anil K. Mishra

[email protected]
| Fax: +91-11-23919509

Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054 India

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Sebastien Lecommandoux,

Corresponding Author

Sebastien Lecommandoux

[email protected]
| Fax: +33-5-4000-8487

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Dipti Kakkar,

Dipti Kakkar

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054 India

Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, 110016 India

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Silvia Mazzaferro,

Silvia Mazzaferro

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Julie Thevenot,

Julie Thevenot

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Christophe Schatz,

Christophe Schatz

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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Anant Bhatt,

Anant Bhatt

Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054 India

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Bilikere S. Dwarakanath,

Bilikere S. Dwarakanath

Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054 India

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Harpal Singh,

Harpal Singh

Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, 110016 India

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Anil K. Mishra,

Corresponding Author

Anil K. Mishra

[email protected]
| Fax: +91-11-23919509

Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054 India

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Sebastien Lecommandoux,

Corresponding Author

Sebastien Lecommandoux

[email protected]
| Fax: +33-5-4000-8487

Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607 Pessac Cedex, France

CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France

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First published: 29 December 2014

https://doi.org/10.1002/mabi.201400451

Citations: 21

Supporting Information is available from the Wiley Online Library or from the author.

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Abstract

Huisgen's 1,3-dipolar cycloaddition (“Click Chemestry”) has been used to prepare amphiphilic PEO-b-PBLG diblock and PBLG-b-PEO-b-PBLG triblock copolymers as potential carriers of anticancer drugs. Spherical and flower shaped micelles (D ≈100 nm) were obtained from diblock and triblock copolymers respectively. DOX was effectively encapsulated up to 18 wt.% and 50–60% of it was steadily released from the micelles over a period of 7 d. Flow cytometry and fluorescence microscopy confirmed the effective intracellular uptake as well as the sustained release of DOX from micelles. These results suggest that the diblock as well as triblock copolymers are promising carriers for intra-cellular drug delivery. mabi201400451-gra-0001

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Citing Literature

Volume15, Issue1

Special Issue:Peptide‐based Materials for Nanomedicine

January 2015

Pages 124-137

Amphiphilic PEO-b-PBLG Diblock and PBLG-b-PEO-b-PBLG Triblock Copolymer Based Nanoparticles: Doxorubicin Loading and In Vitro Evaluation

Abstract

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Amphiphilic PEO-b-PBLG Diblock and PBLG-b-PEO-b-PBLG Triblock Copolymer Based Nanoparticles: Doxorubicin Loading and In Vitro Evaluation

Abstract

Supporting Information

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