Cartilage/bone interface fabricated under perfusion: Spatially organized commitment of adipose-derived stem cells without medium supplementation
Walter Baumgartner
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorLukas Otto
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorSamuel C. Hess
Institute for Chemical- and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland
Search for more papers by this authorWendelin J. Stark
Institute for Chemical- and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland
Search for more papers by this authorSonja Märsmann
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Division of Trauma Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorGabriella Meier Bürgisser
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorMaurizio Calcagni
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorPaolo Cinelli
Division of Trauma Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorCorresponding Author
Johanna Buschmann
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Correspondence to: Johanna Buschmann; e-mail: [email protected]Search for more papers by this authorWalter Baumgartner
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorLukas Otto
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorSamuel C. Hess
Institute for Chemical- and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland
Search for more papers by this authorWendelin J. Stark
Institute for Chemical- and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland
Search for more papers by this authorSonja Märsmann
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Division of Trauma Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorGabriella Meier Bürgisser
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorMaurizio Calcagni
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorPaolo Cinelli
Division of Trauma Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Search for more papers by this authorCorresponding Author
Johanna Buschmann
Division of Plastic and Hand Surgery, University Hospital Zürich, ZKF, Zürich, Switzerland
Correspondence to: Johanna Buschmann; e-mail: [email protected]Search for more papers by this authorAbstract
Tissue engineering of an osteochondral interface demands for a gradual transition of chondrocyte- to osteoblast-prevailing tissue. If stem cells are used as a single cell source, an appropriate cue to trigger the desired differentiation is the use of composite materials with different amounts of calcium phosphate. Electrospun meshes of poly-lactic-co-glycolic acid and amorphous calcium phosphate nanoparticles (PLGA/aCaP) in weight ratios of 100:0; 90:10, 80:20, and 70:30 were seeded with human adipose-derived stem cells (ASCs) and cultured in DMEM without chemical supplementation. After 2 weeks of static cultivation, they were either further cultivated statically for another 2 weeks (group 1), or placed in a Bose® bioreactor with a flow rate per area of 0.16 mL cm−2 min−1 (group 2). Markers for stem cell criteria, chondrogenesis, osteogenesis, adipogenesis and angiogenesis were analyzed by quantitative real-time PCR. Cell distribution, Sox9 protein expression and proteoglycans were assessed by histology. In group 2 (perfusion culture), chondrogenic Sox9 was upregulated toward the cartilage-mimicking side compared to pure PLGA. On the bone-mimicking side, Sox9 experienced a downregulation, which was confirmed on the protein level. Vice versa, expression of osteocalcin was upregulated on the bone-mimicking side, while it was unchanged on the cartilage-mimicking side. In group 1 (static culture), CD31 was upregulated in the presence of aCaP compared to pure PLGA, whereas Sox9 and osteocalcin expression were not affected. aCaP nanoparticles incorporated in electrospun PLGA drive the differentiation behavior of human ASCs in a dose-dependent manner. Discrete gradients of aCaP may act as promising osteochondral interfaces. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1833–1843, 2019.
CONFLICT OF INTEREST
All authors disclose any potential sources of conflict of interest.
Supporting Information
| Filename | Description |
|---|---|
| jbmb34276-sup-0001-Supinfo.docxWord 2007 document , 70.4 KB | Appendix S1: Supporting Information |
| jbmb34276-sup-0002-TableS1.docxWord 2007 document , 13.9 KB | Table S1 The sequences of forward and reverse primers. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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