Detection of fibrotic changes in the progression of liver diseases by label-free multiphoton imaging
Xingxin Huang
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorYuan-E Lian
Department of Pathology, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, China
Search for more papers by this authorLida Qiu
College of Physics and Electronic Information Engineering, Minjiang University, Fuzhou, China
Search for more papers by this authorXunBin Yu
Department of Pathology, Fujian Provincial Hospital, Fuzhou, China
Search for more papers by this authorZhenlin Zhan
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorZheng Zhang
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorXiong Zhang
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorHongxin Lin
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorShuoyu Xu
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China
Search for more papers by this authorJianxin Chen
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorCorresponding Author
Yannan Bai
Shengli Clinical Medical College of Fujian Medical University, Department of Hepatobiliary and Pancreatic Surgery, Fujian Provincial Hospital, Fuzhou, China
Correspondence
Yannan Bai, Department of Hepatobiliopancreatic Surgery, Fujian Provincial Hospital, Fuzhou, 350001, China.
Email: [email protected]
Lianhuang Li, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, 350007, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Lianhuang Li
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Correspondence
Yannan Bai, Department of Hepatobiliopancreatic Surgery, Fujian Provincial Hospital, Fuzhou, 350001, China.
Email: [email protected]
Lianhuang Li, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, 350007, China.
Email: [email protected]
Search for more papers by this authorXingxin Huang
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorYuan-E Lian
Department of Pathology, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, China
Search for more papers by this authorLida Qiu
College of Physics and Electronic Information Engineering, Minjiang University, Fuzhou, China
Search for more papers by this authorXunBin Yu
Department of Pathology, Fujian Provincial Hospital, Fuzhou, China
Search for more papers by this authorZhenlin Zhan
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorZheng Zhang
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorXiong Zhang
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorHongxin Lin
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorShuoyu Xu
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China
Search for more papers by this authorJianxin Chen
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Search for more papers by this authorCorresponding Author
Yannan Bai
Shengli Clinical Medical College of Fujian Medical University, Department of Hepatobiliary and Pancreatic Surgery, Fujian Provincial Hospital, Fuzhou, China
Correspondence
Yannan Bai, Department of Hepatobiliopancreatic Surgery, Fujian Provincial Hospital, Fuzhou, 350001, China.
Email: [email protected]
Lianhuang Li, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, 350007, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Lianhuang Li
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China
Correspondence
Yannan Bai, Department of Hepatobiliopancreatic Surgery, Fujian Provincial Hospital, Fuzhou, 350001, China.
Email: [email protected]
Lianhuang Li, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou, 350007, China.
Email: [email protected]
Search for more papers by this authorXingxin Huang, Yuan-E Lian and Lida Qiu contributed equally to this work.
Abstract
Collagen fibers play an important role in the progression of liver diseases. The formation and progression of liver fibrosis is a dynamic pathological process accompanied by morphological changes in collagen fibers. In this study, we used multiphoton microscopy for label-free imaging of liver tissues, allowing direct detection of various components including collagen fibers, tumors, blood vessels, and lymphocytes. Then, we developed a deep learning classification model to automatically identify tumor regions, and the accuracy reaches 0.998. We introduced an automated image processing method to extract eight collagen morphological features from various stages of liver diseases. Statistical analysis showed significant differences between them, indicating the potential use of these quantitative features for monitoring fibrotic changes during the progression of liver diseases. Therefore, multiphoton imaging combined with automatic image processing method would hold a promising future in rapid and label-free diagnosis of liver diseases.
CONFLICT OF INTEREST STATEMENT
The authors declare that they have no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data supporting the findings of this study are available within the article. The data not shown can be available from the corresponding authors upon reasonable requests.
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