Cetuximab-conjugated nanodiamonds drug delivery system for enhanced targeting therapy and 3D Raman imaging
Dandan Li
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
University of Chinese Academy of Sciences, 100049 PR China
Search for more papers by this authorXin Chen
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
University of Chinese Academy of Sciences, 100049 PR China
School of Pharmaceutical Sciences, Peking University, 100191 PR China
Search for more papers by this authorHong Wang
School of Pharmaceutical Sciences, Peking University, 100191 PR China
Search for more papers by this authorJie Liu
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
Search for more papers by this authorMeiling Zheng
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
Search for more papers by this authorYang Fu
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
University of Chinese Academy of Sciences, 100049 PR China
Search for more papers by this authorCorresponding Author
Yuan Yu
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
Search for more papers by this authorCorresponding Author
Jinfang Zhi
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
Search for more papers by this authorDandan Li
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
University of Chinese Academy of Sciences, 100049 PR China
Search for more papers by this authorXin Chen
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
University of Chinese Academy of Sciences, 100049 PR China
School of Pharmaceutical Sciences, Peking University, 100191 PR China
Search for more papers by this authorHong Wang
School of Pharmaceutical Sciences, Peking University, 100191 PR China
Search for more papers by this authorJie Liu
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
Search for more papers by this authorMeiling Zheng
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
Search for more papers by this authorYang Fu
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
University of Chinese Academy of Sciences, 100049 PR China
Search for more papers by this authorCorresponding Author
Yuan Yu
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
Search for more papers by this authorCorresponding Author
Jinfang Zhi
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 100190 PR China
Search for more papers by this authorAbstract
In this study, a multicomponent nanodiamonds (NDs)-based targeting drug delivery system, cetuximab-NDs-cisplatin bioconjugate, combining both specific targeting and enhanced therapeutic efficacy capabilities, is developed and characterized. The specific targeting ability of cetuximab-NDs-cisplatin system on human liver hepatocellular carcinoma (HepG2) cells is evaluated through epidermal growth factor receptor (EGFR) blocking experiments, since EGFR is over-expressed on HepG2 cell membrane. Besides, cytotoxic evaluation confirms that cetuximab-NDs-cisplatin system could significantly inhibit the growth of HepG2 cells, and the therapeutic activity of this system is proven to be better than that of both nonspecific NDs-cisplatin conjugate and specific EGF-NDs-cisplatin conjugate. Furthermore, a 3-dimensional (3D) Raman imaging technique is utilized to visualize the targeting efficacy and enhanced internalization of cetuximab-NDs-cisplatin system in HepG2 cells, using the NDs existing in the bioconjugate as Raman probes, based on the characteristic Raman signal of NDs at 1332 cm−1. These advantageous properties of cetuximab-NDs-cisplatin system propose a prospective imaging and treatment tool for further diagnostic and therapeutic purposes.
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