The use of optical trap and microbeam to investigate the mechanical and transport characteristics of tunneling nanotubes in tumor spheroids
Corresponding Author
Pooja Patheja
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Corresponding authors: e-mail: [email protected], [email protected]
Search for more papers by this authorCorresponding Author
Raktim Dasgupta
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Department of Theory and Bio-systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany
Corresponding authors: e-mail: [email protected], [email protected]
Search for more papers by this authorAlok Dube
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Search for more papers by this authorSunita Ahlawat
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Search for more papers by this authorRavi Shanker Verma
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Search for more papers by this authorPradeep Kumar Gupta
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Search for more papers by this authorCorresponding Author
Pooja Patheja
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Corresponding authors: e-mail: [email protected], [email protected]
Search for more papers by this authorCorresponding Author
Raktim Dasgupta
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Department of Theory and Bio-systems, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany
Corresponding authors: e-mail: [email protected], [email protected]
Search for more papers by this authorAlok Dube
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Search for more papers by this authorSunita Ahlawat
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Search for more papers by this authorRavi Shanker Verma
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Search for more papers by this authorPradeep Kumar Gupta
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, 452013 India
Search for more papers by this authorAbstract
The use of optical trap and microbeam for investigating mechanical and transport properties of inter cellular tunneling nanotubes (TnTs) in tumor spheroids has been demonstrated. TnTs in tumor spheroids have been visualized by manipulating TnT connected cells using optical tweezers. Functionality of the TnTs for transferring cytoplasmic vesicles and injected dye molecules by optoporation method has been studied. Further, the TnTs could be longitudinally stretched by manipulating the connected cells and their elastic response was studied.
Manipulation of cells at the surface of tumor spheroid using optical tweezers and injection of fluorescent dye into a trapped cell using optoporation technique.
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