Nanoscale distinction of membrane patches – a TERS study of Halobacterium salinarum
Tanja Deckert-Gaudig
Institute of Photonic Technology IPHT, Albert-Einstein-Straße 9, 07745 Jena, Germany
Search for more papers by this authorRené Böhme
Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany
Search for more papers by this authorErik Freier
Lehrstuhl für Biophysik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany
Search for more papers by this authorAleksandar Sebesta
Institute of Photonic Technology IPHT, Albert-Einstein-Straße 9, 07745 Jena, Germany
Search for more papers by this authorTobias Merkendorf
Lehrstuhl für Biophysik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany
Search for more papers by this authorJürgen Popp
Institute of Photonic Technology IPHT, Albert-Einstein-Straße 9, 07745 Jena, Germany
Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany
Search for more papers by this authorKlaus Gerwert
Lehrstuhl für Biophysik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany
Search for more papers by this authorCorresponding Author
Volker Deckert
Institute of Photonic Technology IPHT, Albert-Einstein-Straße 9, 07745 Jena, Germany
Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany
Institute of Photonic Technology (IPHT), Albert-Einstein-Straße 9, 07745 Jena, GermanySearch for more papers by this authorTanja Deckert-Gaudig
Institute of Photonic Technology IPHT, Albert-Einstein-Straße 9, 07745 Jena, Germany
Search for more papers by this authorRené Böhme
Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany
Search for more papers by this authorErik Freier
Lehrstuhl für Biophysik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany
Search for more papers by this authorAleksandar Sebesta
Institute of Photonic Technology IPHT, Albert-Einstein-Straße 9, 07745 Jena, Germany
Search for more papers by this authorTobias Merkendorf
Lehrstuhl für Biophysik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany
Search for more papers by this authorJürgen Popp
Institute of Photonic Technology IPHT, Albert-Einstein-Straße 9, 07745 Jena, Germany
Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany
Search for more papers by this authorKlaus Gerwert
Lehrstuhl für Biophysik, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany
Search for more papers by this authorCorresponding Author
Volker Deckert
Institute of Photonic Technology IPHT, Albert-Einstein-Straße 9, 07745 Jena, Germany
Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany
Institute of Photonic Technology (IPHT), Albert-Einstein-Straße 9, 07745 Jena, GermanySearch for more papers by this authorAbstract
The structural organization of cellular membranes has an essential influence on their functionality. The membrane surfaces currently are considered to consist of various distinct patches, which play an important role in many processes, however, not all parameters such as size and distribution are fully determined. In this study, purple membrane (PM) patches isolated from Halobacterium salinarum were investigated in a first step using TERS (tip-enhanced Raman spectroscopy). The characteristic Raman modes of the resonantly enhanced component of the purple membrane lattice, the retinal moiety of bacteriorhodopsin, were found to be suitable as PM markers. In a subsequent experiment a single Halobacterium salinarum was investigated with TERS. By means of the PM marker bands it was feasible to identify and localize PM patches on the bacterial surface. The size of these areas was determined to be a few hundred nanometers. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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