Quantitative discrimination of NPC cell lines using optical coherence tomography
Jianghua Li
School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China
Search for more papers by this authorCorresponding Author
Changshui Chen
School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China
Phone: 0086+20+85211768, Fax: 0086+20+85211768Search for more papers by this authorBingling Chen
School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China
Search for more papers by this authorZhiyuan Shen
Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
Search for more papers by this authorYonghong He
Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
Search for more papers by this authorYunfei Xia
Department of Radiation Oncology, Cancer Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China
Search for more papers by this authorSonghao Liu
School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China
Search for more papers by this authorJianghua Li
School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China
Search for more papers by this authorCorresponding Author
Changshui Chen
School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China
Phone: 0086+20+85211768, Fax: 0086+20+85211768Search for more papers by this authorBingling Chen
School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China
Search for more papers by this authorZhiyuan Shen
Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
Search for more papers by this authorYonghong He
Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
Search for more papers by this authorYunfei Xia
Department of Radiation Oncology, Cancer Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China
Search for more papers by this authorSonghao Liu
School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China
Search for more papers by this authorAbstract
We tried to explore the intrinsic differences in the optical properties of the four representative NPC cell lines on the models of radiobiology and metastasis by OCT. The scattering coefficients and anisotropies were extracted by fitting the average a-scan attenuation curves based on the multiple scatter effect. The values of scattering coefficients and anisotropy factors were 5.21 ± 0.11, 5.30 ± 0.09, 5.92 ± 0.21, 6.97 ± 0.22, and 0.892 ± 0.009, 0.886 ± 0.006, 0.884 ± 0.009, 0.86 ± 0.01 for CNE1, CNE2, 5-8F and 6-10B pellets (p < 0.05, P = 0.07 for CNE1 and CNE2), respectively. The results showed that the radiobiology and metastasis cell's model could be distinguished obviously; which implied that the corresponding types of NPC tissue might be potentially differentiated by OCT. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
References
- [1] W. I. Wei and J. S. Sham, Lancet 365, 2041–2054 (2005).
- [2] B. Povazay, K. Bizheva, A. Unterhuber, B. Hermann, H. Sallmann, A. F. Fercher, W. Drexler, A. Apolonski, W. J. Wadsworth, J. C. Knight, P. St. J. Russell, M. Vetterlein, and E. Scherzer, Opt. Lett. 27, 1800–1802 (2002).
- [3] R. A. McLaughlin, L. Scolaro, P. Robbins, C. Saunders, S. L. Jacques, and D. D. Sampson, J. Biomed. 15, 046029 (2010).
- [4] M. T. Tsai, C. K. Lee, H. C. Lee, H. M. Chen, C. P. Chiang, Y. M. Wang, and C. C. Yang, J. Biomed. Opt. 14(4), 044028 (2009).
- [5] W. B. Armstrong, J. M. Ridgway, D. E. Vokes, S. Guo, J. Perez, R. P. Jackson, M. Gu, J. Su, R. L. Crumley, T. Y. Shibuya, U. Mahmood, Z. Chen, and B. J. Wong, Phys. Med. Biol. 41, 369–382 (1996).
- [6] E. A. T. Say, S. U. Shah, S. Ferenczy, and C. L. Shields, J. Ophthalmol., DOI 10.1155/2011/385058 (2011).
- [7] A. I. Neugut and S. Pita, Gastroenterology 95(2), 492–499 (1988).
- [8] T. Xie, M. Zeidel, and Yingtian Pan, Opt. Express 10(24), 1431–1443 (2002).
- [9] J. M. Schmitt, A. Knüttel, and R. F. Bonner, Appl. Opt. 32, 6032–6042 (1993).
- [10] D. Levitz, L. Thrane, M. H. Frosz, and P. E. Andersen, Opt. Express 12, 249–259 (2004).
- [11] O. K. Adegun, P. H. Tomlins, E. Hagi-Pavli, G. McKenzie, K. Piper, D. L. Bader, and F. Fortune, Lasers Med. Sci., DOI 10.1007/s10103–011-0975–1 (2011).
- [12] P. H. Tomlins, O. Adegun, E. Hagi-Pavli, K. Piper, D. Bader, and F. Fortune, J. Biomed. Opt. 15(6), 066003 (2010).
- [13] Z. Q. Li, Y. F. Xia, Q. Liu, W. Yi, X. F. Liu, F. Han, W. Luo, and T. X. Lu, Int. J. Radiat. Oncol. Biol. Phys. 66, 1011–1016 (2006).
- [14] H. M. Wang, X. Y. Wu, Y. F. Xia, and J. Y. Qian, Chin. J. Cancer 22, 579–581 (2003).
- [15] L. Jia, F. Ying, C. Jing, K. T. Yao, and Z. X. Huang, Cancer Genet. Cytogenet. 196, 23–30 (2010).
- [16] F. J. Meer, D. J. Faber, M. C. G. Aalders, A. A. Poot, I. Vermes, and T. G. Leeuwen, Lasers Med. Sc. 25(2), 259–267 (2010).
- [17] Z. Y. Shen, M. Wang, Y. H. Ji, Y. H. He, X. S. Dai, P. Li, and H. Ma, Laser Phys. Lett. 8, 318–323, (2011).
- [18] D. Levitz, C. B. Andersen, M. H. Frosz, L. Thrane, P. R. Hansen, T. M. Jørgensen, and P. E. Andersen, Proc. SPIE 5140, 12–19 (2003).
- [19] L. Thrane, H. T. Yura, and P. E. Andersen, J. Opt. Soc. Am. A 17, 484–490 (2000).
- [20]
J. R. Mourant, A. H. Hielscher, A. A. Eick, T. M. Johnson, and J. P. Freyer, Cancer Cytopathol. 84, 366–374 (1998).
10.1002/(SICI)1097-0142(19981225)84:6<366::AID-CNCR9>3.0.CO;2-7 CAS PubMed Web of Science® Google Scholar
- [21] J. R. Mourant, J. P. Freyer, A. H. Hielscher, A. A. Eick, D. Shen, and T. M. Johnson, Appl. Opt. 37, 3586 (1998).
- [22] J. R. Mourant, M. Canpolat, C. Brocker, O. Esponda-Ramos, T. Johnson, A. Matanock, K. Stetter, and J. P. Freyer, J. Biomed. Opt. 5, 131–137 (2000).
- [23] J. R. Mourant, T. M. Johnson, S. Carpenter, A. Guerra, T. Aida, and J. P. Freyer, J. Biomed. Opt. 7(3), 378–387 (2002).
- [24] R. Drezek, A. Dunn, and R. Richards-Kortum, Opt. Express 6, 147–157 (2000), http://www.opticsexpress.org.
- [25] A. M. Sergeev, N. D. Gladkova, F. I. Feldchtein, V. M. Gelikonov, G. V. Gelikonov, L. Snopova, J. Ioannovich, K. Frangia, T. Pirza, I. Antoniou, A. K. Dunn, and R. R. Richards-Kortum, Proc. SPIE 2981, 58–63 (1997).
- [26] B. Mohlenhoff, M. Romeo, M. Diem, and B. R. Wood, Biophys. J. 88(5) 3635–3640 (2005).
- [27] M. Xu and R. R. Alfano, Opt. Lett. 30, 3051–3053 (2005).
- [28] L. C. Junquiera, J. Carneiro, and R. O. Kelley, Basic histology. 7th ed. (Appleton and Lange, Norwalk, 1992).
- [29] T. Xie, M. Zeidel, and Y. Pan, Opt. Express 10, 1431–1443 (2002).
Citing Literature
