Assessing the risk of skin damage due to femtosecond laser irradiation
Frank Fischer
Beiersdorf AG, Unnastraße 48, 20245 Hamburg, Germany
Search for more papers by this authorBeate Volkmer
Dermatologisches Zentrum Buxtehude, Am Krankenhaus 1, 21614 Buxtehude, Germany
Search for more papers by this authorStefan Puschmann
Beiersdorf AG, Unnastraße 48, 20245 Hamburg, Germany
Search for more papers by this authorRüdiger Greinert
Dermatologisches Zentrum Buxtehude, Am Krankenhaus 1, 21614 Buxtehude, Germany
Search for more papers by this authorEckhard Breitbart
Dermatologisches Zentrum Buxtehude, Am Krankenhaus 1, 21614 Buxtehude, Germany
Search for more papers by this authorJürgen Kiefer
Strahlenzentrum, University Gießen, Leihgesterner Weg 217, 35392 Gießen, Germany
Search for more papers by this authorRoger Wepf
Beiersdorf AG, Unnastraße 48, 20245 Hamburg, Germany
Search for more papers by this authorFrank Fischer
Beiersdorf AG, Unnastraße 48, 20245 Hamburg, Germany
Search for more papers by this authorBeate Volkmer
Dermatologisches Zentrum Buxtehude, Am Krankenhaus 1, 21614 Buxtehude, Germany
Search for more papers by this authorStefan Puschmann
Beiersdorf AG, Unnastraße 48, 20245 Hamburg, Germany
Search for more papers by this authorRüdiger Greinert
Dermatologisches Zentrum Buxtehude, Am Krankenhaus 1, 21614 Buxtehude, Germany
Search for more papers by this authorEckhard Breitbart
Dermatologisches Zentrum Buxtehude, Am Krankenhaus 1, 21614 Buxtehude, Germany
Search for more papers by this authorJürgen Kiefer
Strahlenzentrum, University Gießen, Leihgesterner Weg 217, 35392 Gießen, Germany
Search for more papers by this authorRoger Wepf
Beiersdorf AG, Unnastraße 48, 20245 Hamburg, Germany
Search for more papers by this authorAbstract
We irradiated freshly excised skin biopsies with four irradiation regimes usually taken for multiphoton imaging. If there is any skin damaging, it is mainly an effect similar to the damaging effects of UV-irradiation. Using fluorescent antibodies against cyclobutane-pyrimidin-dimers (CPDs) in combination with immuno-fluorescence image analysis we quantitatively compared fs-irradiation effects with UV-irradiation (solar simulator). Based on these results we are giving a risk assessment. The results show that multi photon imaging using the parameters described here is in the ballpark of damaging occurring from every day sun exposure. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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