Zhejiang Agr & For University

Zhejiang Agr & For University

Search term
Advanced Search Citation Search
Search term
Advanced Search Citation Search

Volume 38, Issue 5 pp. 520-526

Article

Full Access

Chromatographic separation of flaxseed phenolics

Dr. R. Amarowicz M. Sc,

Corresponding Author

Dr. R. Amarowicz M. Sc

Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada

Department of Food Sciences, Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, Olsztyn, Poland

Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, CanadaSearch for more papers by this author

P. K. J. P. D. Wanasundara,

Corresponding Author

P. K. J. P. D. Wanasundara

Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada

Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, CanadaSearch for more papers by this author

Prof. Dr. F. Shahidi,

Corresponding Author

Prof. Dr. F. Shahidi

Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada

Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, CanadaSearch for more papers by this author

Dr. R. Amarowicz M. Sc,

Corresponding Author

Dr. R. Amarowicz M. Sc

Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada

Department of Food Sciences, Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, Olsztyn, Poland

Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, CanadaSearch for more papers by this author

P. K. J. P. D. Wanasundara,

Corresponding Author

P. K. J. P. D. Wanasundara

Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada

Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, CanadaSearch for more papers by this author

Prof. Dr. F. Shahidi,

Corresponding Author

Prof. Dr. F. Shahidi

Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada

Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3×9, CanadaSearch for more papers by this author

First published: 1994

https://doi.org/10.1002/food.19940380508

Citations: 17

Share a link

Email
Wechat
Bluesky

Abstract

A column chromatography method for separation of phenolic compounds from flaxseed using Sephadex LH-20, RP-8 and silica gel is described. TLC analysis proved that separated fractions contained numerous phenolic compounds. All fractions exhibited a maximum UV absorption between 270 and 290 nm. Additional shoulders were observed between 274 and 350 nm. These results imply that lignans and their derivatives are the main phenolic compounds present in flaxseed.

Abstract

Chromatographische Trennung von Flachssamen-Phenolen

Es wird eine säulenchromatographische Methode zur Trennung von phenolischen Verbindungen von Flachssamen unter Verwendung von Sephadex LH-20, RP-8 und Silicagel beschrieben. TLC-Analysen zeigen, daß die getrennten Fraktionen zahlreiche phenolische Verbindungen enthalten. Alle Fraktionen weisen ein Maximum der UV-Absorption zwischen 270 und 290 nm auf, zusätzliche Schultern werden zwischen 274 und 350 nm beobachtet. Die Ergebnisse lassen darauf schließen, daß Lignane und ihre Derivate die wesentlichen Phenolverbindungen im Flachssamen sind.

References

1 Amarowicz, R., H. Kozlowska, M. Shimoyamada and K. Okubo, Pol. J. Food Nutr. Sci. 1 (1992) 128.
Google Scholar
2 Amarowicz, R., U. Wanasundara, P. K. J. P. D. Wanasundara and F. Shahidi, J. Food Lipids 1 (1993) 111.
10.1111/j.1745-4522.1993.tb00239.x
CAS Google Scholar
3 Axelson, M., J. Sjovall, B. E. Gustafsson and K. D. Setchel, Nature 298 (1982) 659.
10.1038/298659a0
CAS PubMed Web of Science® Google Scholar
4 Barton, G. M., R. S. Evans and J. A. F. Gardner, Nature 170 (1952) 249.
10.1038/170249a0
CAS Web of Science® Google Scholar
5 Bekke, J. E., and H. J. Klosterman, Proc. North. Dacota Acad. Sci. 10 (1956).
Google Scholar
6 Conte, L. S., G. Lecker, P. Capella and M. Catena, Rivisita Italiana Della Sostanze Grasse 56 (1979) 339.
CAS Google Scholar
7 Dabrowski, K. J., and F. W. Sosulski, J. Agric. Food Chem. 32 (1984) 128.
10.1021/jf00121a032
CAS Web of Science® Google Scholar
8 Dubois, M., K. A. Gilles, J. K. Reber and P. A. Smith, Anal. Chem. 28 (1956) 350.
10.1021/ac60111a017
CAS Web of Science® Google Scholar
9 Dybing, C. D., and C. Lay, in: Handbook of Biosolar Resources, Vol. II. Ed. by O. R. Zabroskey, pp. 71–85. CRC Press, New York 1981.
Google Scholar
10 Hamilton, R. J., in: Recent Advances in Chemistry and Technology of Fats and Oils. Ed. by R. J. Hamilton and A. Bhati, pp. 109–166. Elsevier, London and New York 1987.
10.1007/978-94-011-7471-8_7
Web of Science® Google Scholar
11 Holub, B. J., Flax Inst. US 53 (1990) 12.
Google Scholar
12 Jing, Z., D. U. Ahn and J. S. Sim, Poult. Sci. 70 (1991) 2467.
10.3382/ps.0702467
PubMed Web of Science® Google Scholar
13 Kozlowska, H., R. Zadernowski and F. W. Sosulski, Nahrung 27 (1983) 449.
10.1002/food.19830270517
CAS PubMed Web of Science® Google Scholar
14 Naczk, M., and F. Shahidi, Food Chem. 40 (1989) 159.
10.1016/0308-8146(89)90026-5
CAS Web of Science® Google Scholar
15 Naczk, M., P. K. J. P. D. Wanasundara and F. Shahidi, J. Agric. Food Chem. 40 (1992) 444.
10.1021/jf00015a016
CAS Web of Science® Google Scholar
16 Wanasundara, P. K. J. P. D., R. Amarowicz, M. T. Kara and F. Shahidi, Food Chem. 48 (1993) 263.
10.1016/0308-8146(93)90138-6
CAS Web of Science® Google Scholar
17 Wanasundara, P. K. J. P. D., and F. Shahidi, Food Chem. 49 (1993) 39.
10.1016/0308-8146(94)90230-5
CAS Web of Science® Google Scholar

Citing Literature

Volume38, Issue5

1994

Pages 520-526

The full text of this article hosted at iucr.org is unavailable due to technical difficulties.