Highly Sensitive Fluorometric Assay Method for Acetylcholinesterase Inhibitor Based on Nile Red-Adsorbed Gold Nanoparticles
Wenting Han
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorShuzhen Liao
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorChonghua Zhang
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorHuazhi Ding
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorCorresponding Author
Zhaoyang Wu
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China, Tel. 0086-0731-88821989Search for more papers by this authorGuoli Shen
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorRuqin Yu
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorWenting Han
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorShuzhen Liao
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorChonghua Zhang
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorHuazhi Ding
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorCorresponding Author
Zhaoyang Wu
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China, Tel. 0086-0731-88821989Search for more papers by this authorGuoli Shen
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorRuqin Yu
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China
Search for more papers by this authorAbstract
A new sensitive fluorometric assay method for acetylcholinesterase (AChE) and its inhibitor was developed using a fluorescent dye, nile red (NR). Due to the fluorescence resonance energy transfer between the NR and the gold nanoparticle (AuNPs), the fluorescence was quenched. AChE can break down acetylthiocholine to produce a thiol-bearing compound, thiocholine. In the presence of thiocholine, the nile red is replaced from the AuNPs surfaces and simultaneously transformed to a derivative of nile red. The fluorescence intensity of the derivative is much stronger than that of the native nile red with the same concentration and its maximum emission wavelength has a blue shift so that the sensor achieves a good signal-to-background ratio. In addition, when organophosphate pesticide (OPs) exists, the activity of AChE can be inhibited, the generation of thiocholine will be prevented and no fluorescence enhancement occurs. The results show that the method is sensitive to AChE and paraoxon with the detection limits of 0.2 mU/mL and 0.05 ng/mL, respectively.
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