Functionalized Magnetizable Particles for Downstream Processing in Single-Use Systems
Dr. Nils Tippkötter
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, Germany
Search for more papers by this authorHuschyar Al-Kaidy
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, Germany
Search for more papers by this authorSteffen Wollny
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, Germany
Search for more papers by this authorCorresponding Author
Prof. Dr. Roland Ulber
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, Germany
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, GermanySearch for more papers by this authorDr. Nils Tippkötter
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, Germany
Search for more papers by this authorHuschyar Al-Kaidy
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, Germany
Search for more papers by this authorSteffen Wollny
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, Germany
Search for more papers by this authorCorresponding Author
Prof. Dr. Roland Ulber
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, Germany
University of Kaiserslautern, Institute of Bioprocess Engineering, Gottlieb-Daimler-Straße 44, 67663 Kaiserslautern, GermanySearch for more papers by this authorAbstract
Biotechnological downstream processing is usually an elaborate procedure, requiring a multitude of unit operations to isolate the target component. Besides the disadvantageous space-time yield, the risks of cross-contaminations and product loss grow fast with the complexity of the isolation procedure. A significant reduction of unit operations can be achieved by application of magnetic particles, especially if these are functionalized with affinity ligands. As magnetic susceptible materials are highly uncommon in biotechnological processes, target binding and selective separation of such particles from fermentation or reactions broths can be done in a single step. Since the magnetizable particles can be produced from iron salts and low priced polymers, a single-use implementation of these systems is highly conceivable. In this article, the principles of magnetizable particles, their synthesis and functionalization are explained. Furthermore, applications in the area of reaction engineering, microfluidics and downstream processing are discussed focusing on established single-use technologies and development potential.
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