Regular Article
Unusual Temperature-Induced Retention Behavior of Constrained β-Amino Acid Enantiomers on the Zwitterionic Chiral Stationary Phases ZWIX(+) and ZWIX(–)
István Ilisz,
Zoltán Pataj,
Zsanett Gecse,
Zsolt Szakonyi,
Ferenc Fülöp,
Wolfgang Lindner,
Antal Péter,
István Ilisz
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorZoltán Pataj
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorZsanett Gecse
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary
Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorZsolt Szakonyi
Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorFerenc Fülöp
Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorWolfgang Lindner
Department of Analytical Chemistry, University of Vienna, Vienna, Austria
Search for more papers by this authorAntal Péter
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorIstván Ilisz,
Zoltán Pataj,
Zsanett Gecse,
Zsolt Szakonyi,
Ferenc Fülöp,
Wolfgang Lindner,
Antal Péter,
István Ilisz
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorZoltán Pataj
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorZsanett Gecse
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary
Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorZsolt Szakonyi
Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorFerenc Fülöp
Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorWolfgang Lindner
Department of Analytical Chemistry, University of Vienna, Vienna, Austria
Search for more papers by this authorAntal Péter
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary
Search for more papers by this authorCorrespondence to: Antal Péter, Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, H-6720 Szeged, Hungary. E-mail: [email protected]
ABSTRACT
The effects of temperature on the chiral recognition of cyclic β-amino acid enantiomers on zwitterionic [Chiralpak ZWIX(+) and ZWIX(–)] chiral stationary phases were investigated. Experiments were performed at different mobile phase compositions and under 10°C column temperature increments in the temperature range 10–50°C. Apparent thermodynamic parameters and Tiso values were calculated from plots of ln k and ln α versus 1/T, respectively. Unusual temperature behavior was observed, especially on the ZWIX(–) column, where the application of MeOH/MeCN (50/50 v/v) containing 25 mM triethylamine and 50 mM formic acid as mobile phase led to nonlinear van't Hoff plots and increasing retention time with increasing temperature. On both columns, both enthalpically and entropically driven separations were observed. Chirality 26:385–393, 2014. © 2014 Wiley Periodicals, Inc.
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