Partitioning Studies of Glutaminase in Polyethylene Glycol and Salt-Based Aqueous Two-Phase Systems
S. Bolar
National Institute of Technology Karnataka, Department of Chemical Engineering, Mangalore, India
Search for more papers by this authorP. D. Belur
National Institute of Technology Karnataka, Department of Chemical Engineering, Mangalore, India
Search for more papers by this authorCorresponding Author
R. Iyyaswami
National Institute of Technology Karnataka, Department of Chemical Engineering, Mangalore, India
National Institute of Technology Karnataka, Department of Chemical Engineering, Mangalore, IndiaSearch for more papers by this authorS. Bolar
National Institute of Technology Karnataka, Department of Chemical Engineering, Mangalore, India
Search for more papers by this authorP. D. Belur
National Institute of Technology Karnataka, Department of Chemical Engineering, Mangalore, India
Search for more papers by this authorCorresponding Author
R. Iyyaswami
National Institute of Technology Karnataka, Department of Chemical Engineering, Mangalore, India
National Institute of Technology Karnataka, Department of Chemical Engineering, Mangalore, IndiaSearch for more papers by this authorAbstract
The partitioning behavior of glutaminase produced from Zygosaccharomyces rouxii in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPSs) was investigated. ATPSs comprising of different PEG-salts were considered. Binodal data and tie lines generated for the selected systems were analyzed and correlated with Othmer-Tobias and Bancroft equations. Effects of salt type, PEG molecular weight, concentrations of phase components, and tie line length on enzyme partitioning were evaluated.
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