Cover Picture: Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy (Angew. Chem. Int. Ed. 52/2016)
Graphical Abstract
Cytoplasmic re-targeting of the pyrrolysine-based genetic code expansion machinery by using a nuclear export signal (NES) substantially increases the site-specific incorporation of clickable amino acids (green) into proteins in mammalian cells, as shown by E. A. Lemke and co-workers in their Communication on page 16172 ff. These clickable amino acids can then be modified with annealing sites for small DNA imaging strands (red) to enable high-contrast Click-PAINT super-resolution microscopy.
Cytoplasmic re-targeting of the pyrrolysine-based genetic code expansion machinery by using a nuclear export signal (NES) substantially increases the site-specific incorporation of clickable amino acids (green) into proteins in mammalian cells, as shown by E. A. Lemke and co-workers in their Communication on page 16172 ff. These clickable amino acids can then be modified with annealing sites for small DNA imaging strands (red) to enable high-contrast Click-PAINT super-resolution microscopy.
Zeolitic Imidazolate Frameworks
Silanides
Rhodium Single-Atom Catalysts
