Activation and Retention: A Magnetic Resonance Probe for the Detection of Acute Thrombosis†
The National Institutes of Health are acknowledged for funding to P.C. (HL109448)) and G.S.L. (CA009502) and instrumentation grants (RR023385, EB015896). Chris Farrar is thanked for help with the MRI experiment.
Graphical Abstract
Identify new blood clots: The enzyme protein disulfide isomerase (PDI) plays an important role in facilitating the aggregation of activated platelets during the formation of new blood clots. By modifying a key disulfide bridge in the fibrin imaging probe EP-2104R, a new activatable magnetic resonance probe has been prepared that is responsive to PDI and therefore has the potential to be used for the identification of nascent blood clots.
Abstract
Blood-clot formation that results in the complete occlusion of a blood vessel (thrombosis) often leads to serious life-threatening events, such as strokes and heart attacks. As the composition of a thrombus changes as it matures, new imaging methods that are capable of distinguishing new clots from old clots may yield important diagnostic and prognostic information. To address this need, an activatable magnetic resonance (MR) probe that is responsive to a key biochemical process associated with recently formed clots has been developed.
