A Suite of Solid-State NMR Experiments for RNA Intranucleotide Resonance Assignment in a 21 kDa Protein–RNA Complex†
Dr. Alexander Marchanka
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany)
Search for more papers by this authorDr. Bernd Simon
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany)
Search for more papers by this authorCorresponding Author
Dr. Teresa Carlomagno
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany)
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany)===Search for more papers by this authorDr. Alexander Marchanka
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany)
Search for more papers by this authorDr. Bernd Simon
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany)
Search for more papers by this authorCorresponding Author
Dr. Teresa Carlomagno
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany)
Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany)===Search for more papers by this authorThis work was supported by the DFG (grant number CA294/3-1), by the BMBF though project 0315870 and by the EMBL. We thank Prof. Bernd Reif (TU, Munich) for useful discussions.
Graphical Abstract
Intranucleotide resonance of the 26mer box C/D RNA in complex with the L7Ae protein were assigned by solid-state NMR (ssNMR; see picture) spectroscopy. This investigation opens the way for studying RNA in large protein–RNA complexes by ssNMR spectroscopy.
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