A Synthetic Riboswitch that Operates using a Rationally Designed Ligand–RNA Pair†
Corresponding Author
Dr. Chikara Dohno
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
PRESTO (Japan) Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012 (Japan)
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)===Search for more papers by this authorIzumi Kohyama
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
Search for more papers by this authorMaki Kimura
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
Search for more papers by this authorDr. Masaki Hagihara
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
Graduate School of Science and Technology, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561 (Japan)
Search for more papers by this authorCorresponding Author
Prof. Kazuhiko Nakatani
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)===Search for more papers by this authorCorresponding Author
Dr. Chikara Dohno
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
PRESTO (Japan) Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012 (Japan)
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)===Search for more papers by this authorIzumi Kohyama
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
Search for more papers by this authorMaki Kimura
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
Search for more papers by this authorDr. Masaki Hagihara
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
Graduate School of Science and Technology, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561 (Japan)
Search for more papers by this authorCorresponding Author
Prof. Kazuhiko Nakatani
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)===Search for more papers by this authorThis work was supported by Grant in Aid for Scientific Research on Innovative Areas (21200042) from the Japan Society for the Promotion of Science (JSPS) and Sumitomo Foundation for C.D. and Grant in Aid for Scientific Research (A) (23241073) and the Advanced research for medical products Mining Program of the National Institute of Biomedical Innovation (10–22) for K.N.
Graphical Abstract
The construction of an artificial riboswitch is based on a ligand–RNA pair without any molecular biology-based selection processes. The ligand selectively and significantly stabilized an RNA duplex containing an r(XGG)/r(XGG) sequence (X=U, A, G). The integration of the ligand-binding sequences into the 5′-untranslated region of mRNA provided an artificial riboswitch that was responsive to Z-NCTS.
Supporting Information
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