Chemistry as an Expanding Resource in Protein Science: Fully Synthetic and Fully Active Human Parathyroid Hormone-Related Protein (1–141)†
Dr. Jianfeng Li
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
Search for more papers by this authorDr. Suwei Dong
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
Search for more papers by this authorDr. Steven D. Townsend
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
Search for more papers by this authorDr. Thomas Dean
Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, 51 Blossom Street, Boston, MA (USA)
Search for more papers by this authorProf. Thomas J. Gardella
Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, 51 Blossom Street, Boston, MA (USA)
Search for more papers by this authorCorresponding Author
Prof. Samuel J. Danishefsky
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027 (USA)
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)Search for more papers by this authorDr. Jianfeng Li
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
Search for more papers by this authorDr. Suwei Dong
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
Search for more papers by this authorDr. Steven D. Townsend
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
Search for more papers by this authorDr. Thomas Dean
Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, 51 Blossom Street, Boston, MA (USA)
Search for more papers by this authorProf. Thomas J. Gardella
Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, 51 Blossom Street, Boston, MA (USA)
Search for more papers by this authorCorresponding Author
Prof. Samuel J. Danishefsky
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)
Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027 (USA)
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065 (USA)Search for more papers by this authorThis research was supported by NIH grant HL25848 (S.J.D.). S.D.T. is grateful to Weill Cornell Medical School for an NIH postdoctoral fellowship (CA062948).
Graphical Abstract
The convergent synthesis of human parathyroid hormone-related protein (hPTHrP, see scheme) has been accomplished through iterative peptide ligations followed by global metal-free dethiylation. The biological activity of synthetic PTHrP has been demonstrated.
Supporting Information
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