Angewandte Chemie International Edition in English

Communication

Full Access

A “Smart” Magnetic Resonance Imaging Agent That Reports on Specific Enzymatic Activity^†

Rex A. Moats

The Beckman Institute, 139-74, California Institute of Technology, Pasadena, CA 91125 (USA), Fax: Int. code +(818)449-5163, e-mail: [email protected]

Search for more papers by this author

Scott E. Fraser,

Scott E. Fraser

The Beckman Institute, 139-74, California Institute of Technology, Pasadena, CA 91125 (USA), Fax: Int. code +(818)449-5163, e-mail: [email protected]

Search for more papers by this author

Dr. Thomas J. Meade,

Corresponding Author

Dr. Thomas J. Meade

The Beckman Institute, 139-74, California Institute of Technology, Pasadena, CA 91125 (USA), Fax: Int. code +(818)449-5163, e-mail: [email protected]

The Beckman Institute, 139-74, California Institute of Technology, Pasadena, CA 91125 (USA), Fax: Int. code +(818)449-5163, e-mail: [email protected]Search for more papers by this author

Rex A. Moats,

Rex A. Moats

The Beckman Institute, 139-74, California Institute of Technology, Pasadena, CA 91125 (USA), Fax: Int. code +(818)449-5163, e-mail: [email protected]

Search for more papers by this author

Scott E. Fraser,

Scott E. Fraser

The Beckman Institute, 139-74, California Institute of Technology, Pasadena, CA 91125 (USA), Fax: Int. code +(818)449-5163, e-mail: [email protected]

Search for more papers by this author

Dr. Thomas J. Meade,

Corresponding Author

Dr. Thomas J. Meade

The Beckman Institute, 139-74, California Institute of Technology, Pasadena, CA 91125 (USA), Fax: Int. code +(818)449-5163, e-mail: [email protected]

The Beckman Institute, 139-74, California Institute of Technology, Pasadena, CA 91125 (USA), Fax: Int. code +(818)449-5163, e-mail: [email protected]Search for more papers by this author

First published: April 18, 1997

https://doi.org/10.1002/anie.199707261

Citations: 306

^†

This research was supported by the Baxter Foundation, the Beckman Institute, and the NIH (AR42671). The authors thank Eric Ahrens and Russell Jacobs for helpful discussions and for assistance with the acquisition of MR images.

About

PDF

Tools

Share a link

Email
Wechat
Bluesky

Graphical Abstract

A noninvasive means to map biological structure is offered by magnetic resonance imaging (MRI). A new class of MRI contrast agents is described where the relaxivity of the complex is modified by the activity of a specific enzyme (β-galactosidase, [Eq. (a)]). This type of agent offers the promise of direct three-dimensional visualization of gene expression in the form of an acquired MR image.

References

1 R. Y. Tsien, ACS Symp. Ser. 1993, 538, 130–146.
10.1021/bk-1993-0538.ch009
CAS Web of Science® Google Scholar
2 H. E. Keller in Handbook of Biological Confocal Microscopy, 2nd ed. (Ed.: J. B. Pawley), Plenum, New York, 1995, p. 111.
10.1007/978-1-4757-5348-6_7
Google Scholar
3 R. W. Bowtell, J. C. Sharp, P. Mansfield, E. W. Hsu, N. Aiken, A. Horsman, Mag. Res. in Med. 1995, 33, 790–794;
10.1002/mrm.1910330608
CAS PubMed Web of Science® Google Scholar
C. T. Rofe, J. Vannoort, P. J. Back, P. T. Callaghan, J. Mag. Res. Ser. B. 1995, 108, 125–136;
10.1006/jmrb.1995.1113
CAS Web of Science® Google Scholar
Z. P. Liang, P. C. Lauterbur, IEEE Trans. Med. Imaging 1994, 13, 677–686;
10.1109/42.363100
CAS PubMed Web of Science® Google Scholar
R. E. Jacobs, S. E. Fraser, Science 1994, 263, 681;
10.1126/science.7508143
CAS PubMed Web of Science® Google Scholar
A. F. Mellin, G. P. Cofer, B. R. Smith, S. A. Suddarth, L. W. Hedlund, G. A. Johnson, Mag. Reson. Med. 1994, 32, 199–205;
10.1002/mrm.1910320208
CAS PubMed Web of Science® Google Scholar
W. Kuhn, Angew. Chem. 1990, 29, 1–19.
Google Scholar
4 I. Bertini, C. Luchinat, NMR of Paramagnetic Molecules in Biological Systems, Benjamin/Cummings, 1986.
Google Scholar
5 S. H. Koenig, K. E. Kellar, Magn. Res. Med. 1995, 34, 227–233.
10.1002/mrm.1910340214
CAS PubMed Web of Science® Google Scholar
6 V. Alexander, Chem. Rev. 1995, 95, 273–242;
10.1021/cr00034a002
CAS Web of Science® Google Scholar
K. Kumar, C. A. Chang, M. F. Tweedle, Inorg. Chem. 1993, 32, 587–593.
10.1021/ic00057a017
CAS Web of Science® Google Scholar
7 L. S. Szczepaniak, A. Sargeson, I. I. Creasei, R. J. Geue, M. F. Tweedle, R. G. Bryant, Bioconjuagate 1992, 3, 27–31.
10.1021/bc00013a004
CAS PubMed Web of Science® Google Scholar
8 K. Kumar, M. F. Tweedle, Pure Appl. Chem. 1993, 65, 515–520.
10.1351/pac199365030515
CAS Web of Science® Google Scholar
9 S. I. Kang, R. S. Ranganathan, J. E. Emswiler, K. Kumar, J. Z. Gougoutas, M. F. Malley, M. F. Tweedle, Inorg. Chem. 1993, 32, 2912–2918;
10.1021/ic00065a019
CAS Web of Science® Google Scholar
S. Aime, M. Botta, G. Ermondi, Inorg. Chem. 1992, 31, 4291–4299.
10.1021/ic00047a016
CAS Web of Science® Google Scholar
10 M. Li, P. R. Selvin, J. Am. Chem. Soc. 1995, 117, 8132–8138.
10.1021/ja00136a010
CAS Web of Science® Google Scholar
11 W. D. Horrocks, D. R. Sudnick, J. Am. Chem. Soc. 1979, 101, 334–339.
10.1021/ja00496a010
CAS Web of Science® Google Scholar
12 X. Zhang, C. A. Chang, H. G. Brittain, J. M. Garrison, J. M. Telser, M. F. Tweedle, Inorg. Chem. 1992, 31, 5597–5600.
10.1021/ic00052a040
CAS Web of Science® Google Scholar
13 The molar quantity related to these T₁ values is the relaxivity R. R values at 500 MHz were determined for EGad (1800 mM s⁻¹) and Gad (2400 mMs⁻¹ mMs⁻¹) and compared to that of a related species, DO3a (2700 mMs⁻¹).
Google Scholar
14 J. F. Kayyem, R. M. Kumar, S. E. Fraser, T. J. Meade, Chem. and Bio. 1995, 2, 615–620.
10.1016/1074-5521(95)90126-4
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume36, Issue7

April 18, 1997

Pages 726-728

A “Smart” Magnetic Resonance Imaging Agent That Reports on Specific Enzymatic Activity^†

Graphical Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

A “Smart” Magnetic Resonance Imaging Agent That Reports on Specific Enzymatic Activity†

Graphical Abstract

References

Citing Literature

References

Related

Information

A “Smart” Magnetic Resonance Imaging Agent That Reports on Specific Enzymatic Activity^†