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Titelbild: Entschlüsselung der Polymer-Interaktionen in Biokonjugaten unterschiedlicher Architektur durch zeitaufgelöste limitierte Proteolyse mit massenspektrometrischer Analyse (Angew. Chem. 9/2025)
First published: 10 February 2025
Graphical Abstract
Limited proteolysis mass spectrometry allows for in deep molecular characterization of complex bioconjugates. In their Communication (e202415354), Lorenz Meinel, Tessa Lühmann et al. present a protocol for time-resolved proteolysis, deciphering interaction sites within protein–protein complexes and protein–polymer bioconjugates by 15N labeling. The cover image showcases the proteolysis of interferon-α2a and interferon-bioconjugates, resulting in peptide fragments that are analyzed using mass spectrometry.
