A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator
Corresponding Author
Dr. Seiichi Uchiyama
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorCorresponding Author
Dr. Toshikazu Tsuji
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004 Japan
Search for more papers by this authorKyoko Kawamoto
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorDr. Kentaro Okano
Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, 980-8578 Japan
Department of Chemical Science and Engineering, Kobe University, Kobe, Hyogo, 657-8501 Japan
Search for more papers by this authorEiko Fukatsu
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorTakahiro Noro
Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, 980-8578 Japan
Search for more papers by this authorKumiko Ikado
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004 Japan
Search for more papers by this authorSayuri Yamada
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004 Japan
Search for more papers by this authorYuka Shibata
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorDr. Teruyuki Hayashi
The Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan
Department of Nutrition, Koshien University, 10-1 Momijigaoka, Takarazuka, Hyogo, 665-0006 Japan
Search for more papers by this authorDr. Noriko Inada
The Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan
Search for more papers by this authorDr. Masaru Kato
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorDr. Hideki Koizumi
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004 Japan
Search for more papers by this authorCorresponding Author
Prof. Hidetoshi Tokuyama
Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, 980-8578 Japan
Search for more papers by this authorCorresponding Author
Dr. Seiichi Uchiyama
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorCorresponding Author
Dr. Toshikazu Tsuji
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004 Japan
Search for more papers by this authorKyoko Kawamoto
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorDr. Kentaro Okano
Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, 980-8578 Japan
Department of Chemical Science and Engineering, Kobe University, Kobe, Hyogo, 657-8501 Japan
Search for more papers by this authorEiko Fukatsu
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorTakahiro Noro
Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, 980-8578 Japan
Search for more papers by this authorKumiko Ikado
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004 Japan
Search for more papers by this authorSayuri Yamada
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004 Japan
Search for more papers by this authorYuka Shibata
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorDr. Teruyuki Hayashi
The Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan
Department of Nutrition, Koshien University, 10-1 Momijigaoka, Takarazuka, Hyogo, 665-0006 Japan
Search for more papers by this authorDr. Noriko Inada
The Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192 Japan
Search for more papers by this authorDr. Masaru Kato
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 Japan
Search for more papers by this authorDr. Hideki Koizumi
Central Laboratories for Key Technologies, KIRIN Company Limited, 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa, 236-0004 Japan
Search for more papers by this authorCorresponding Author
Prof. Hidetoshi Tokuyama
Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, 980-8578 Japan
Search for more papers by this authorAbstract
A cationic fluorescent nanogel thermometer based on thermo-responsive N-isopropylacrylamide and environment-sensitive benzothiadiazole was developed with a new azo compound bearing imidazolium rings as the first cationic radical initiator. This cationic fluorescent nanogel thermometer showed an excellent ability to enter live mammalian cells in a short incubation period (10 min), a high sensitivity to temperature variations in live cells (temperature resolution of 0.02–0.84 °C in the range 20–40 °C), and remarkable non-cytotoxicity, which permitted ordinary cell proliferation and even differentiation of primary cultured cells.
Supporting Information
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