Molecular Imaging of Biological Samples on Nanophotonic Laser Desorption Ionization Platforms
Sylwia A. Stopka
Department of Chemistry, The George Washington University, Washington, DC, 20052 USA
Search for more papers by this authorCharles Rong
Department of Chemistry, The George Washington University, Washington, DC, 20052 USA
Search for more papers by this authorDr. Andrew R. Korte
Department of Chemistry, The George Washington University, Washington, DC, 20052 USA
Search for more papers by this authorDr. Sridevi Yadavilli
Research Center for Genetic Medicine, Children's National Medical Center, Washington, DC, 2001 USA
Search for more papers by this authorDr. Javad Nazarian
Research Center for Genetic Medicine, Children's National Medical Center, Washington, DC, 2001 USA
Search for more papers by this authorDr. Trust T. Razunguzwa
Protea Biosciences Inc., Morgantown, WV, 26505 USA
Search for more papers by this authorDr. Nicholas J. Morris
Protea Biosciences Inc., Morgantown, WV, 26505 USA
Search for more papers by this authorCorresponding Author
Prof. Akos Vertes
Department of Chemistry, The George Washington University, Washington, DC, 20052 USA
Search for more papers by this authorSylwia A. Stopka
Department of Chemistry, The George Washington University, Washington, DC, 20052 USA
Search for more papers by this authorCharles Rong
Department of Chemistry, The George Washington University, Washington, DC, 20052 USA
Search for more papers by this authorDr. Andrew R. Korte
Department of Chemistry, The George Washington University, Washington, DC, 20052 USA
Search for more papers by this authorDr. Sridevi Yadavilli
Research Center for Genetic Medicine, Children's National Medical Center, Washington, DC, 2001 USA
Search for more papers by this authorDr. Javad Nazarian
Research Center for Genetic Medicine, Children's National Medical Center, Washington, DC, 2001 USA
Search for more papers by this authorDr. Trust T. Razunguzwa
Protea Biosciences Inc., Morgantown, WV, 26505 USA
Search for more papers by this authorDr. Nicholas J. Morris
Protea Biosciences Inc., Morgantown, WV, 26505 USA
Search for more papers by this authorCorresponding Author
Prof. Akos Vertes
Department of Chemistry, The George Washington University, Washington, DC, 20052 USA
Search for more papers by this authorAbstract
Mass spectrometry imaging (MSI) is a comprehensive tool for the analysis of a wide range of biomolecules. The mainstream method for molecular MSI is matrix-assisted laser desorption ionization, however, the presence of a matrix results in spectral interferences and the suppression of some analyte ions. Herein we demonstrate a new matrix-free MSI technique using nanophotonic ionization based on laser desorption ionization (LDI) from a highly uniform silicon nanopost array (NAPA). In mouse brain and kidney tissue sections, the distributions of over 80 putatively annotated molecular species are determined with 40 μm spatial resolution. Furthermore, NAPA-LDI-MS is used to selectively analyze metabolites and lipids from sparsely distributed algal cells and the lamellipodia of human hepatocytes. Our results open the door for matrix-free MSI of tissue sections and small cell populations by nanophotonic ionization.
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