Spontaneous Reconstitution of Functional Transmembrane Proteins During Bioorthogonal Phospholipid Membrane Synthesis
Christian M. Cole
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.edu
Search for more papers by this authorDr. Roberto J. Brea
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.edu
Search for more papers by this authorYoung Hun Kim
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Pacific Hall 6160, La Jolla, CA 92093 (USA)
Search for more papers by this authorMichael D. Hardy
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.edu
Search for more papers by this authorProf. Jerry Yang
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Pacific Hall 6160, La Jolla, CA 92093 (USA)
Search for more papers by this authorCorresponding Author
Prof. Neal K. Devaraj
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.edu
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.eduSearch for more papers by this authorChristian M. Cole
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.edu
Search for more papers by this authorDr. Roberto J. Brea
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.edu
Search for more papers by this authorYoung Hun Kim
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Pacific Hall 6160, La Jolla, CA 92093 (USA)
Search for more papers by this authorMichael D. Hardy
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.edu
Search for more papers by this authorProf. Jerry Yang
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Pacific Hall 6160, La Jolla, CA 92093 (USA)
Search for more papers by this authorCorresponding Author
Prof. Neal K. Devaraj
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.edu
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Building: Urey Hall 4120, La Jolla, CA 92093 (USA) http://devarajgroup.ucsd.eduSearch for more papers by this authorAbstract
Transmembrane proteins are critical for signaling, transport, and metabolism, yet their reconstitution in synthetic membranes is often challenging. Non-enzymatic and chemoselective methods to generate phospholipid membranes in situ would be powerful tools for the incorporation of membrane proteins. Herein, the spontaneous reconstitution of functional integral membrane proteins during the de novo synthesis of biomimetic phospholipid bilayers is described. The approach takes advantage of bioorthogonal coupling reactions to generate proteoliposomes from micelle-solubilized proteins. This method was successfully used to reconstitute three different transmembrane proteins into synthetic membranes. This is the first example of the use of non-enzymatic chemical synthesis of phospholipids to prepare proteoliposomes.
Supporting Information
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
| Filename | Description |
|---|---|
| ange_201504339_sm_miscellaneous_information.pdf1.4 MB | miscellaneous_information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
References
- 1
- 1aM. S. Almén, K. J. Nordström, R. Fredriksson, H. B. Schiöth, BMC Biol. 2009, 7, 50–63;
- 1bN. Gamper, M. S. Shapiro, Nat. Rev. Neurosci. 2007, 8, 921–934.
- 2
- 2aJ. L. Maître, C. P. Heisenberg, Curr. Biol. 2013, 23, R 626–R633;
- 2bI. D. Campbell, M. J. Humphries, Cold Spring Harbor Perspect. Biol. 2011, 3, a 004994.
- 3J. P. Overington, B. Al-Lazikani, A. L. Hopkins, Nat. Rev. Drug Discovery 2006, 5, 993–996.
- 4G. S. Hotamisligil, Nature 2006, 444, 860–867.
- 5D. J. Selkoe, Physiol. Rev. 2001, 81, 741–766.
- 6C. G. Nichols, G. K. Singh, D. K. Grange, Circ. Res. 2013, 112, 1059–1072.
- 7J.-L. Rigaud, B. Pitard, D. Levy, Biochim. Biophys. Acta Bioenerg. 1995, 1231, 223–246.
- 8A. M. Seddon, P. Curnow, P. J. Booth, Biochim. Biophys. Acta Biomembr. 2004, 1666, 105–117.
- 9M. Baker, Nat. Methods 2010, 7, 429–434.
- 10
- 10aE. M. Sletten, C. R. Bertozzi, Acc. Chem. Res. 2011, 44, 666–676;
- 10bN. A. McGrath, R. T. Raines, Acc. Chem. Res. 2011, 44, 752–761;
- 10cJ. C. Jewett, C. R. Bertozzi, Chem. Soc. Rev. 2010, 39, 1272–1279.
- 11
- 11aM. D. Hardy, J. Yang, J. Selimkhanov, C. M. Cole, L. S. Tsimring, N. K. Devaraj, Proc. Natl. Acad. Sci. USA 2015, 112, 8187–8192;
- 11bI. Budin, N. K. Devaraj, J. Am. Chem. Soc. 2012, 134, 751–753.
- 12R. J. Brea, C. M. Cole, N. K. Devaraj, Angew. Chem. Int. Ed. 2014, 53, 14102–14105; Angew. Chem. 2014, 126, 14326–14329.
- 13Copper catalyzed azide–alkyne cycloaddition (CuAAC):
- 13aJ. E. Hein, V. V. Fokin, Chem. Soc. Rev. 2010, 39, 1302–1315;
- 13bC. W. Tornøe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057–3064;
- 13cV. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. Int. Ed. 2002, 41, 2596–2599;
10.1002/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO;2-4 CAS PubMed Web of Science® Google ScholarAngew. Chem. 2002, 114, 2708–2711.
- 14Native chemical ligation (NCL):
- 14aO. Vázquez, O. Seitz, J. Pept. Sci. 2014, 20, 78–86;
- 14bL. Raibaut, N. Ollivier, O. Melnyk, Chem. Soc. Rev. 2012, 41, 7001–7015;
- 14cP. E. Dawson, T. W. Muir, I. Clark-Lewis, S. B. H. Kent, Science 1994, 266, 776–779.
- 15S. Yoshikawa, K. Muramoto, K. Shinzawa-Itoh, M. Mochizuki, Biochim. Biophys. Acta Bioenerg. 2012, 1817, 579–589.
- 16S. Yoshikawa, K. Muramoto, K. Shinzawa-Itoh, Biochim. Biophys. Acta Bioenerg. 2011, 1807, 1279–1286.
- 17S. B. Vik, R. A. Capaldi, Biochemistry 1977, 16, 5755–5759.
- 18L. Smith, H. Conrad, Arch. Biochem. Biophys. 1956, 63, 403–413.
- 19P. Rosevear, T. VanAken, J. Baxter, S. Ferguson-Miller, Biochemistry 1980, 19, 4108–4115.
- 20J.-L. Rigaud, G. Mosser, J.-J. Lacapere, A. Olofsson, D. Levy, J.-L. Ranck, J. Struct. Biol. 1997, 118, 226–235.
- 21R. M. Straubinger, D. Papahadjopoulos, K. Hong, Biochemistry 1990, 29, 4929–4939.
- 22
- 22aR. Doshi, A. Ali, W. Shi, E. V. Freeman, L. A. Fagg, H. W. van Veen, J. Biol. Chem. 2013, 288, 6801–6813;
- 22bP. D. Eckford, F. J. Sharom, J. Biol. Chem. 2008, 283, 12840–12850;
- 22cD. Y. Haubertin, H. Madaoui, A. Sanson, R. Guerois, S. Orlowski, Biophys. J. 2006, 91, 2517–2531.
- 23
- 23aC. Pinet, S. Antoine, A. G. Filoteo, J. T. Penniston, A. Coulombe, J. Membr. Biol. 2002, 187, 185–201;
- 23bS. Antoine, C. Pinet, A. Coulombe, J. Membr. Biol. 2001, 179, 37–50.
- 24D. A. Zacharias, C. Kappen, Biochim. Biophys. Acta Gen. Subj. 1999, 1428, 397–405.
- 25E. E. Strehler, D. A. Zacharias, Physiol. Rev. 2001, 81, 21–50.
- 26C. G. Tate, FEBS Lett. 2001, 504, 94–98.
- 27M. C. Chicka, E. E. Strehler, J. Biol. Chem. 2003, 278, 18464–18470.
- 28G. M. Fang, H. K. Cui, J. S. Zheng, L. Liu, ChemBioChem 2010, 11, 1061–1065.
- 29
- 29aM. S. Khan, N. S. Dosoky, J. D. Williams, Int. J. Mol. Sci. 2013, 14, 21561–21597;
- 29bR. S. Ries, H. Choi, R. Blunck, F. Bezanilla, J. R. Heath, J. Phys. Chem. B 2004, 108, 16040–16049.
- 30
- 30aR. Capone, S. Blake, M. Rincon Restrepo, J. Yang, M. Mayer, J. Am. Chem. Soc. 2007, 129, 9737–9745;
- 30bS. Blake, T. Mayer, M. Mayer, J. Yang, ChemBioChem 2006, 7, 433–435.
- 31B. Hille, Ion Channels of Excitable Membranes, 3rd ed., Sinauer Associates, Inc., Sunderland (USA), 2001, 7–11.
- 32
- 32aS. Majd, E. C. Yusko, Y. N. Billeh, M. X. Macrae, J. Yang, M. Mayer, Curr. Opin. Biotechnol. 2010, 21, 439–476;
- 32bM. Montal, P. Mueller, Proc. Natl. Acad. Sci. USA 1972, 69, 3561–3566.
- 33T. Lefevre, E. Coraboeuf, A. Ghazi, A. Coulombe, J. Membr. Biol. 1995, 147, 147–158.
Citing Literature
This is the
German version
of Angewandte Chemie.
Note for articles published since 1962:
Do not cite this version alone.
Take me to the International Edition version with citable page numbers, DOI, and citation export.
We apologize for the inconvenience.
